Rapamycin 别名：Sirolimus; Rapamune; AY-22989; 雷帕霉素

目录号 M1768

Rapamycin (Sirolimus)是一种选择性的mTOR的大环内酯类抑制剂，能通过抑制mTOR激酶活性，从而激活ULK1复合物，导致自噬（autophagy）水平的增加，其IC50值为0.1 nM。

CAS No.：53123-88-9

规格	价格	库存状态
Free Sample (0.5-1 mg)	¥ 0	现货
10mM*1mL in DMSO	¥ 480	现货
5mg	¥ 300	现货
10mg	¥ 455	现货
25mg	¥ 546	现货
50mg	¥ 650	现货
100mg	¥ 845	现货
200mg	¥ 1300	现货

*AbMole所有产品仅供有资质的科研机构或医药企业进行科学研究或药证申报用途，不能被用于人体和任何其它用途。我们不向任何个人或非科研性质的机构提供产品和服务。

客户使用AbMole的Rapamycin发表的文献

Cell Metab. 2024 Oct 18.

Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex

Biomed Pharmacother. 2024 Mar;172:116260..

Panax notoginseng saponins stimulates the differentiation and neurite development of C17.2 neural stem cells against OGD/R injuries via mTOR signaling

Mol Carcinog. 2024 Jul 30.

SIRT1 silencing promotes EMT and Crizotinib resistance by regulating autophagy through AMPK/mTOR/S6K signaling pathway in EML4-ALK L1196M and EML4-ALK G1202R mutant non-small cell lung cancer cells

Discover Oncology. 2024 Jun 15.

Lycorine attenuated proliferation and induced apoptosis on imatinib-resistant K562 cell by inhibiting autophagy

Adv Sci (Weinh). 2023 Mar 27;e2207224.

Hypoxia Drives Material‐Induced Heterotopic Bone Formation by Enhancing Osteoclastogenesis via M2/Lipid‐Loaded Macrophage Axis

Metabolism. 2023 Jan 7;140:155398.

Metformin coordinates with mesenchymal cells to promote VEGF-mediated angiogenesis in diabetic wound healing through Akt/mTOR activation

Front Immunol. 2023 Mar 14;14:1120996.

ROS-AMPK/mTOR-dependent enterocyte autophagy is involved in the regulation of Giardia infection-related tight junction protein and nitric oxide levels

Commun Biol. 2023 Apr 3;6(1):361.

WSSV exploits AMPK to activate mTORC2 signaling for proliferation by enhancing aerobic glycolysis

Infect Immun. 2023 Nov 16;91(11):e0010323.

Effects of Trichinella spiralis and its serine protease inhibitors on autophagy of host small intestinal cells

J Orthop Surg Res. 2023 Feb 22;18(1):129.

Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy

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Arch Oral Biol. 2023 Apr 18;151:105700.

Wrinkled topography regulates osteogenesis via autophagy-mediated Wnt/β-catenin signaling pathway in MC3T3-E1 cells

Mol Cancer. 2022 Mar 18;21(1):77.

Cholesterol promotes EGFR-TKIs resistance in NSCLC by inducing EGFR/Src/Erk/SP1 signaling-mediated ERRα re-expression

J Hepatol. 2022 Nov 9;S0168-8278(22)03285-8.

Hepatocyte-specific Mas activation enhances lipophagy and fatty acid oxidation to protect against acetaminophen-induced hepatotoxicity in mice

Mol Psychiatry. 2022 Jul 15;1-14.

Social isolation reinforces aging-related behavioral inflexibility by promoting neuronal necroptosis in basolateral amygdala

Immunology. 2022 Sep 25.

mTORC1-GLUT1-mediated glucose metabolism drives hyperactivation of B cells in primary Sjogren's syndrome

CNS Neurosci Ther. 2022 Nov 24.

Acupuncture promotes nerve repair through the benign regulation of mTOR-mediated neuronal autophagy in traumatic brain injury rats

Cell Cycle. 2022 Sep 19;1-23.

Mechanistic target of rapamycin (mTOR) regulates self-sustained quiescence, tumor indolence and late clinical metastasis in a Beclin-1-dependent manner.

Exp Cell Res. 2022 Nov 9;422(1):113414.

Gastric cancer-derived mesenchymal stem cells promote gastric cancer cell lines migration by modulating CD276 expression

Oxid Med Cell Longev. 2021 Jun 14;2021:6662225.

Impaired Autophagy Induced by oxLDL/ β 2GPI/anti- β 2GPI Complex through PI3K/AKT/mTOR and eNOS Signaling Pathways Contributes to Endothelial Cell Dysfunction

Int J Case Rep Orthop. 2021;3(2):129-134.

Rapamycin's osteogenic effects on bone marrow mesenchymal stem cells through triggering autophagy

Oncogene. 2020 Apr;39(15):3163-3178.

The EGFR-ZNF263 Signaling Axis Silences SIX3 in Glioblastoma Epigenetically

Cell Commun Signal. 2020 Feb 17;18(1):27.

mTOR May Interact With PARP-1 to Regulate Visible Light-Induced Parthanatos in Photoreceptors

Mol Immunol. 2020 Jan;117:12-19.

Toll-like receptor 4 knockout protects against diabetic-induced imbalance of bone metabolism via autophagic suppression.

Biomed Res Int. 2020 Jun 20;2020:9243681.

Shenmai Injection Supresses Glycolysis and Enhances Cisplatin Cytotoxicity in Cisplatin-Resistant A549/DDP Cells via the AKT-mTOR-c-Myc Signaling Pathway

Genes & Diseases. 2020 Jun.

Metformin ameliorates HMGB1-mediated oxidative stress through mTOR pathway in experimental periodontitis

Biosci Rep. 2018 Nov 20;38(6).

Rapamycin attenuates mitochondrial injury and renal tubular cell apoptosis in experimental contrast-induced acute kidney injury in rats.

BMC Genomics. 2017 Sep 11;18(1):714.

The dynamic landscape of gene regulation during Bombyx mori oogenesis

Oncotarget. 2016 Feb 22.

Mechanosensitive caveolin-1 activation-induced PI3K/Akt/mTOR signaling pathway promotes breast cancer motility, invadopodia formation and metastasis in vivo.

Mol Med Rep. 2016 Sep;14(3):2194-8.

Anti‑migratory effect of rapamycin impairs allograft imaging by 18F‑fluorodeoxyglucose‑labeled splenocytes

APMIS. 2015 Jul;562-70.

MicroRNA-218 increases cellular sensitivity to Rapamycin via targeting Rictor in cervical cancer.

2015 Apr.

THE MECHANOBIOLOGICAL MECHANISMS OF CAVEOLAE/CAVEOLIN-1 IN LOW SHEAR STRESS-INDUCED BREAST CARCINOMA CELL MIGRATION AND INVASION

质量标准及产品资料

纯度：99.55%
COA
H-NMR
HPLC

化学性质/溶解性/储存

分子量	914.18
分子式	C51H79NO13
CAS号	53123-88-9
中文名称	雷帕霉素；西罗莫司
溶解性	DMSO 80 mg/mL
储存条件	粉末型式 -20°C 3年；4°C 2年溶于溶剂 -80°C 6个月；-20°C 1个月
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

生物活性

Rapamycin (Sirolimus)是一种选择性的mTOR抑制剂，IC50为~0.1 nM。Rapamycin作用于HEK293细胞，抑制内源性mTOR活性，IC50为~0.1 nM,而iRap和AP21967 作用时，IC50分别为~5 nM 和~10 nM。 Rapamycin处理酿酒酵母，诱导细胞周期停在G1/S期，且抑制翻译。 Rapamycin显著抑制T98G和U87-MG细胞活力，这种作用具有剂量依赖性，IC50分别为2 nM和 1 μM, 而对U373-MG细胞没有作用活性，IC50>25 μM。

实验参考

蛋白/细胞实验

下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	1.0939 mL	5.4694 mL	10.9388 mL
5 mM	0.2188 mL	1.0939 mL	2.1878 mL
10 mM	0.1094 mL	0.5469 mL	1.0939 mL

*吸湿的DMSO对产品的溶解度有显著影响，请使用新开封的DMSO；
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

质量		浓度		体积		分子量*
	=		x		x

细胞系	HeLa, SiHa, Caski, and C33A cells
方法	MTT assay. In brief, 2*103 cells/well were seeded into 96-well plates and routinely cultured overnight. Then, these cells were treated with Rapamycin for 72 h. Next, 5 µL MTT (5 mg/ mL; Sigma-Aldrich) and 200 µL DMSO were sequentially added into each well and the absorbance was measured at the wavelength of 570 nm.
浓度	0~80nM
处理时间	72 h

* 上述方法来自公开文献，仅供相同目的实验参考。如实验目的、材料、方法不同，请参考其他文献。

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。

动物实验方案计算器

请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。

1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。

动物模型	cervical cancer C33A cells tumor bearing mice
配制	a solution of 0.2%CMC and 0.25% Tween-80 in ddH2O
剂量	2.5 mg/kg from the 10th day to 37th day
给药处理	intra-peritoneal injection