3-Methyladenine (3-MA) 别名：3-MA; 3-甲基腺嘌呤

目录号 M2296

3-Methyladenine (3-MA)是一种选择性PI3K抑制剂，作用于Vps34和PI3Kγ，IC50分别为25 μM和60 μM；永久抑制I型PI3K，但对III型PI3K的抑制是短暂的，也抑制自噬体的形成。现配现用

CAS No.：5142-23-4

规格	价格	库存状态
Free Sample (0.5-1 mg)	¥ 0	现货
25mg	¥ 324	现货
50mg	¥ 450	现货
100mg	¥ 678	现货
200mg	¥ 1224	现货
500mg	¥ 2394	现货

*AbMole所有产品仅供有资质的科研机构或医药企业进行科学研究或药证申报用途，不能被用于人体和任何其它用途。我们不向任何个人或非科研性质的机构提供产品和服务。

客户使用AbMole的3-Methyladenine (3-MA)发表的文献

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Dihydromyricetin alleviates inflammatory bowel disease associated intestinal fibrosis by inducing autophagy through the PI3K/AKT/mTOR signaling pathway

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Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy

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Effects of autophagy inhibitor 3-methyladenine on a diabetic mice model

CNS Neurosci Ther. 2022 Nov 24.

Acupuncture promotes nerve repair through the benign regulation of mTOR-mediated neuronal autophagy in traumatic brain injury rats

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J Ethnopharmacol. 2022 Sep 15;295:115398.

Yangjing Zhongyu decoction facilitates mitochondrial activity, estrogenesis, and energy metabolism in H2O2-induced human granulosa cell line KGN

Evid Based Complement Alternat Med. 2022 Apr 6;2022:4445293.

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Oxid Med Cell Longev. 2021 Jun 14;2021:6662225.

Impaired Autophagy Induced by oxLDL/ β 2GPI/anti- β 2GPI Complex through PI3K/AKT/mTOR and eNOS Signaling Pathways Contributes to Endothelial Cell Dysfunction

BMC Cancer. 2021 Feb 25;21(1):195.

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Int J Case Rep Orthop. 2021;3(2):129-134.

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Resveratrol Sensitizes Carfilzomib-Induced Apoptosis via Promoting Oxidative Stress in Multiple Myeloma Cells

Mol Immunol. 2017 Jul;87:132-140.

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质量标准及产品资料

纯度：99.90%
COA
H-NMR
HPLC

化学性质/溶解性/储存

分子量	149.15
分子式	C6H7N5
CAS号	5142-23-4
中文名称	3-甲基腺嘌呤
溶解性	Water 5 mg/mL warmed
储存条件	粉末型式 -20°C 3年；4°C 2年溶于溶剂 -80°C 6个月；-20°C 1个月
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

生物活性

3-Methyladenine是一种选择性PI3K抑制剂，作用于Vps34和PI3Kγ，IC50分别为25 μM和60 μM；永久抑制I型PI3K，但对III型PI3K的抑制是短暂的，也抑制自噬体的形成。3-MA对Vps34轻微的偏向性可能是由于Vps34中某个特异性的疏水环形结构可以环绕在3-MA的3-甲基基团外面造成的。据报道对于正常培养和饥饿处理的癌细胞3-MA都能引起细胞死亡。3-MA还可以不通过抑制自吞噬来抑制细胞迁移和入侵，这表明3-MA 除了抑制自吞噬之外还有其它生物功能。现配现用

实验参考

蛋白/细胞实验

下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	6.7047 mL	33.5233 mL	67.0466 mL
5 mM	1.3409 mL	6.7047 mL	13.4093 mL
10 mM	0.6705 mL	3.3523 mL	6.7047 mL

*吸湿的DMSO对产品的溶解度有显著影响，请使用新开封的DMSO；
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

质量		浓度		体积		分子量*
	=		x		x

细胞系	U251 cells
方法	Western blotting was performed to determine the expression levels of ubiquitinated proteins, PDI and Grp78. The expression levels of ubiquitinated PDI and Grp78 were increased following the inhibition of autophagy by treatment with 3-MA
浓度	10 mM
处理时间	12 h

* 上述方法来自公开文献，仅供相同目的实验参考。如实验目的、材料、方法不同，请参考其他文献。

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。