Ferrostatin-1 铁抑素-1

生物活性

Ferrostatin-1 (Fer-1)是一种有效的选择性的ferroptosis抑制剂，抑制Erastin诱导的HT-1080细胞铁死亡，EC50为60 nM。Ferrostatin-1(2 μM)作用于癌细胞，抑制Erastin诱导的Ferroptosis，Ferroptosis是一种铁依赖性的的非凋亡性细胞死亡，Ferrostatin-1作用于新生大鼠脑片，抑制 谷氨酸盐诱导的细胞死亡。Ferrostatin-1是一种脂质ROS清除剂，具有N-环己基部分，作为生物膜内亲脂锚。Ferrostatin-1还是一种抗氧化剂，通过还原机制来防止膜脂的损伤，从而抑制细胞死亡。Ferrostatin-1也具有抗真菌活性。

使用AbMole产品发表的文献

• J Hazard Mater. 2024 Jul 15;476:135211.

  Neurodevelopmental toxicity of a ubiquitous disinfection by-product, bromoacetic acid, in Zebrafish (Danio rerio)

• Chemosphere. 2024 Jul 16.

  Indeno[1,2,3-cd]pyrene enhances the sensitivity of airway epithelial cells to ferroptosis and aggravates asthma

• J Agric Food Chem. 2024 Jul 24;72(31):17356-17367.

  Herpotrichone A Exerts Neuroprotective Effects by Relieving Ferroptosis

• biochemistry and molecular biology. 2024 May.

  MDH2 Promotes Hepatocellular Carcinoma Growth through Ferroptosis Evasion via Stabilizing GPX4

• Biomolecules. 2024 Nov 05.

  Iberverin Downregulates GPX4 and SLC7A11 to Induce Ferroptotic Cell Death in Hepatocellular Carcinoma Cells

• Toxicol Appl Pharmacol. 2024 Jan;116825.

  Anti-NOTCH1 therapy with OMP-52 M51 inhibits salivary adenoid cystic carcinoma by depressing epithelial-mesenchymal transition (EMT) process and inducing …

• Oxid Med Cell Longev. 2023 Jan 14;2023:6896790.

  Curcumin Induces Ferroptosis in Follicular Thyroid Cancer by Upregulating HO-1 Expression

• Cancer Cell Int. 2023 Jan 13;23(1):5.

  Heat shock protein 27 deficiency promotes ferrous ion absorption and enhances acyl-Coenzyme A synthetase long-chain family member 4 stability to promote

• Apoptosis. 2023 Apr;28(3-4):607-626.

  Inhibition of ferroptosis by icariin treatment attenuates excessive ethanol consumption-induced atrial remodeling and susceptibility to atrial fibrillation, role of SIRT1

• J Cancer Res Clin Oncol. 2023 Apr 29.

  Apatinib combined with olaparib induces ferroptosis via a p53-dependent manner in ovarian cancer

• Oxid Med Cell Longev. 2022 Apr 16;2022:4505513.

  Nrf2-Mediated Ferroptosis Inhibition Exerts a Protective Effect on Acute-on-Chronic Liver Failure

• Food Funct. 2022 Oct 3;13(19):10210-10224.

  Vitamin D3 Attenuates Cisplatin-Induced Intestinal Injury by Inhibiting Ferroptosis, Oxidative Stress, and ROS-mediated Excessive Mitochondrial Fission

• Food Chem Toxicol. 2022 Jun;164:113046.

  DEHP induces ferroptosis in testes via p38α-lipid ROS circulation and destroys the BTB integrity

• Anat Rec (Hoboken). 2020 Jan 21.

  Resibufogenin inhibited colorectal cancer cell growth and tumorigenesis through triggering ferroptosis and ROS production mediated by GPX4 inactivation

实验参考

体外实验*
细胞系	BEAS-2B cells
方法	Cell viability assay In brief, BEAS-2B cells were seeded into a 96-well plate at the concentration of 5 × 104 cells/well. The cells were cultured for 24 h, then treated with LPS (Sigma) and Fer-1 (Sigma) in different concentrations for 16 h followed by the addition of 20 μl of CCK-8 solution directly into the medium (200 μl per well) and incubation at 37 °C for 4 h. The absorbances (Abs) in different groups were detected at 450 nm (n = 3). In the blank group, the well only contained medium, and the cells without any treatment were used as the control group. Herein, the cell viability = (Abs of experimental group-Abs of blank group)/(Abs of control group-Abs of blank group) × 100%.
浓度	2 μM
处理时间	16 h

*上述方法来自公开文献，仅供相同目的实验参考。如实验目的、材料、方法不同，请参考其他文献。

体内实验*
动物模型	male C57BL/6 mice
配制	The Fer-1 was dissolved in DMSO first, and diluted with 0.9% NaCl. The final concentration of Fer-1 and DMSO was 0.2 mg/ml and 0.1% respectively.
剂量	0.8 mg/kg
给药处理	via tail vein injection

*上述方法来自公开文献，仅供相同目的实验参考。如实验目的、材料、方法不同，请参考其他文献。

化学性质

分子量	262.35
分子式	C15H22N2O2
CAS号	347174-05-4
溶解性（25°C）	DMSO ≥ 50 mg/mL
储存条件	-20°C, protect from light, dry, sealed
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

储备液配制

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.8117 mL	19.0585 mL	38.117 mL
5 mM	0.7623 mL	3.8117 mL	7.6234 mL
10 mM	0.3812 mL	1.9059 mL	3.8117 mL

溶液配置摩尔浓度计算器

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的Km系数
	动物 A的Km系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

参考文献

[1] Wanting Shu, et al. Redox Biol. Ferrous but not ferric iron sulfate kills photoreceptors and induces photoreceptor-dependent RPE autofluorescence

[2] Pengfei Liu, et al. Cell Mol Biol Lett. Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis