重组 DNase I (RNase Free)

产品描述

DNase I是一种通过重组表达纯化获得的，非特异性的核酸内切酶，并且在纯化过程中去除RNase活性。可以降解染色体以及单链和双链的DNA，并能通过水解磷酸二酯键，产生具有 5 '-磷酸基和3 '-羟基的单核苷酸和寡核苷酸，从而发挥作用。该酶发挥活性需要Mg2+或Ca2+离子的参与，最适pH约为7.8。

用途

（1）体外转录后去除模板DNA

（2）去除RNA中DNA的污染

优势

（1）无RNase活性

（2）纯度高，可直接用于扩增反应

信息

（1）存储液：10mM Tris-HCl,2mM CaCl2，50%甘油，pH=7.6

（2）储存温度：-20°C

使用AbMole产品发表的文献

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参考文献

[1] Olga V Morozova, et al. Int J Mol Sci. Stable Enzymatic Nanoparticles from Nucleases, Proteases, Lipase and Antioxidant Proteins with Substrate-Binding and Catalytic Properties

[2] Stanislas Goriely, et al. Blood. Human IL-12(p35) gene activation involves selective remodeling of a single nucleosome within a region of the promoter containing critical Sp1-binding sites

[3] J Boyes, et al. J Mol Biol. Perturbation of nucleosome structure by the erythroid transcription factor GATA-1

[4] P Bauer, et al. Biotechniques. Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion

[5] W J Lukiw, et al. Brain Res Mol Brain Res. Protein-DNA interactions in the promoter region of the amyloid precursor protein (APP) gene in human neocortex