Actinomycin D 放线菌素D

生物活性

Actinomycin D（RASP-101）是一种从土壤细菌中分离出来的多肽抗生素。Actinomycin D可抑制DNA的修复和RNA的转录、转运，使细胞周期停滞在G1期。Actinomycin D是自噬的激活剂，可在淋巴细胞性白血病中诱导p53依赖性细胞的死亡。

使用AbMole产品发表的文献

• Adv Sci (Weinh). 2023 Jan;10(2):e2201724.

  Silencing of IRF8 Mediated by m6A Modification Promotes the Progression of T‐Cell Acute Lymphoblastic Leukemia

• J Hazard Mater. 2023 May 6;455:131543.

  BPDE exposure promotes trophoblast cell pyroptosis and induces miscarriage by up-regulating lnc-HZ14/ZBP1/NLRP3 axis

• Plant Biotechnol J. 2023 Mar 22.

  N6‐methyladenosine RNA modification regulates cotton drought response in a Ca2+ and ABA‐dependent manner

• J Exp Clin Cancer Res. 2023 May 19;42(1):127.

  Epigenetically silenced lncRNA SNAI3-AS1 promotes ferroptosis in glioma via perturbing the m6A-dependent recognition of Nrf2 mRNA mediated by SND1

• Plant Cell. 2023 Jun 27;koad189.

  N4-acetylation of cytidine in (m) RNA plays essential roles in plants

• Int J Biol Sci. 2023 Jan 1;19(2):691-704.

  Upregulation of LRRC8A by m5C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer

• Antiviral Res. 2023 Jan;209:105482.

  Ciliatoside A, isolated from Peristrophe japonica, inhibits HBsAg expression and cccDNA transcription by inducing autophagy

• Sci Total Environ. 2023 Sep 18;167202.

  m6A methylation-mediated PGC-1α contributes to ferroptosis via regulating GSTK1 in arsenic-induced hepatic insulin resistance

• Cell Death Dis. 2023 Sep 21;14(9):619.

  Interfering with Rac1-activation during neonatal monocyte-macrophage differentiation influences the inflammatory responses of M1 macrophages

• Front Immunol. 2023 Mar 30;14:1144774.

  LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma

• J Transl Med. 2023 Jun 23;21(1):407.

  METTL3-mediated m6A modification of SIRT1 mRNA inhibits progression of endometriosis by cellular senescence enhancing

• Biomed Pharmacother. 2023 May;161:114575.

  Targeting the TNF-α–TNFR interaction with EGCG to block NF-κB signaling in human synovial fibroblasts

• Clin Epigenetics. 2023 Jul 5;15(1):109.

  FTO-dependent m6A regulates muscle fiber remodeling in an NFATC1–YTHDF2 dependent manner

• Clin Epigenetics. 2023 Jul 5;15(1):109.

  FTO-dependent m6A regulates muscle fiber remodeling in an NFATC1-YTHDF2 dependent manner

• J Cell Biochem. 2023 Feb;124(2):239-253.

  IGF2BP2 enhances LincRNA01116 stability via m6A: A potential biomarker and therapeutic target for patients with pre‐eclampsia

• BMC Complementary Medicine and Therapies. 2023 Jul 1.

  NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression

• J Orthop Sci. 2023 May 27;S0949-2658(23)00133-1.

  CircBBS9 accelerates the malignant progression of osteosarcoma through sponging miR-485-3p/HMGB1 axis

• Mol Cancer. 2022 Feb 14;21(1):49.

  Exosomal circLPAR1 functions in colorectal cancer diagnosis and tumorigenesis through suppressing BRD4 via METTL3–eIF3h interaction

• Cells. 2022 Sep 27;11(19):3008.

  m6A Modification of Long Non-Coding RNA HNF1A-AS1 Facilitates Cell Cycle Progression in Colorectal Cancer via IGF2BP2-Mediated CCND1 mRNA Stabilization

• Cell Biol Toxicol. 2022 Feb 28;1-17.

  AS3MT facilitates NLRP3 inflammasome activation by m6A modification during arsenic-induced hepatic insulin resistance

• iScience. 2022 Nov 10;25(12):105552.

  Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

• Molecules. 2022 May 16;27(10):3182.

  A Novel Strategy for Regulating mRNA's Degradation via Interfering the AUF1's Binding to mRNA

• Cancer Lett. 2021 Dec 28;523:135-147.

  IGFBP7-AS1 is a p53-responsive long noncoding RNA downregulated by Epstein-Barr virus that contributes to viral tumorigenesis

• Theranostics. 2020 Apr 27;10(13):5671-5686.

  SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma

产品使用成果展示

	数据来源	Theranostics (2020 Apr). Figure 5. Actinomycin D (Abmole Bioscience, US)
	方法	RNA decay assay
	细胞系/动物模型	MHCC97H and HepG2 cells
	浓度	5 μg/mL
	处理时间	-
	实验结果	We next sought to assess the RNA decay rate in Mettl3-WT and Mettl3-KR cells treated with the transcription inhibitor actinomycin D to block new RNA synthesis. Snail mRNA expression was decreased, and the Snail mRNA half-lives were significantly shortened in SUMO-mutant Mettl3 cells

化学性质

分子量	1255.41
分子式	C62H86N12O16
CAS号	50-76-0
溶解性（25°C）	DMSO ≥ 30 mg/mL
储存条件	粉末型式       -20°C   3年；4°C   2年 溶于溶剂       -80°C   6个月；-20°C   1个月
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

储备液配制

*下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	0.7966 mL	3.9828 mL	7.9655 mL
5 mM	0.1593 mL	0.7966 mL	1.5931 mL
10 mM	0.0797 mL	0.3983 mL	0.7966 mL

不同实验动物依据体表面积的等效剂量转换表（参考来源于公开文献）

	小鼠	大鼠	兔	豚鼠	仓鼠	狗
重量 (kg)	0.02	0.15	1.8	0.4	0.08	10
体表面积 (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km 系数	3	6	12	8	5	20

动物 A (mg/kg) = 动物 B (mg/kg) ×	动物 B的Km系数
	动物 A的Km系数

例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的K_m系数（3），再除以大鼠的K_m系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。

参考文献

[1] Heinicke U,et.al. Oncotarget. JNJ-26481585 primes rhabdomyosarcoma cells for chemotherapeutics by engaging the mitochondrial pathway of apoptosis.

[2] Anthony A Cuneo, et al. J Mol Cell Cardiol. Il-19 reduces VSMC activation by regulation of mRNA regulatory factor HuR and reduction of mRNA stability

[3] Elisabetta Bini, et al. RNA. Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus

[4] S Li, et al. Toxicol Appl Pharmacol. Superinduction of IL-2 gene expression by vomitoxin (deoxynivalenol) involves increased mRNA stability