产品优势:
组分清晰:8 种抑制剂按照固定浓度配比,分独立两支包装,组分之间互不影响。
稳定高效:防止蛋白和脂类的磷酸化状态丢失,产出率较高,即用型溶液形式提供。
物美价廉:质量保障同时,价格合理,为您节省研究开支。
实验数据:
产品描述:
本产品是由8 种独立的磷酸酶抑制剂(不含EDTA) 按照优化的配比配置而成的磷酸酶抑制剂混合物。分为A、B 两管:A 管中是磷酸根类似物,以阻止研究中蛋白和脂类的磷酸化状态丢失;B 管则是新兴的天然产物,为蛋白磷酸化提供更加全面的保护。
使用方法:
1. 本产品适用于保护哺乳动物细胞及组织中蛋白和脂类磷酸化的稳定性。可用于蛋白免疫印迹(Western Blot), 免疫共沉淀(Co-IP),免疫荧光(IF) ,免疫组织化学(IHC),激酶测定(kinase assay)和抗体、酶诊断试剂盒(Dignose Kit)等实验。
2. 实验时,按照1:100 的容积比,将本品预先加入在已准备好的实验体系中,先加A 管,混匀;再加B 管,再次混匀。
保存条件:
本产品应于-20℃环境下密封保存,产品有效期为1 年。使用时可短时间放置于常温或4℃中,用毕应立即放回-20℃中保存。
注意事项:
1. 本产品不含EDTA, 为100×H2O 和100×DMSO 储存液形式。使用时应调整浓度为1×。
2. 本产品置于-20℃保存时,DMSO 呈现冰晶状态,此属于正常现象,非产品质量问题。若B 管融化时间较长,可在25℃水浴中融化。
3. A.B 两管不可以同时加入,以防止沉淀形成。必须先加A 管,再加B 管。
4. 若想得到较为完整的蛋白,推荐使用Abmole 蛋白酶抑制剂(Protease Inhibotor Cocktail)。
5. 本产品仅供科研使用。
常见问题解答:
1. B 管为什么融化较慢?
回答: 因为B 管溶解在DMSO 中,而它储存在-20℃中会结为冰晶,使用时,为了不影响实验进行,需在25℃中水浴融化。
2. A 管、B 管可以一起使用吗?
回答: 不可以。两支混在一起会形成绿色沉淀,使用时需分开加入,先加A 管,后加B 管。
3. 为什么做Western Blot 时得到的磷酸化和非磷酸化的条带都非常弱?
回答: Phophatase Cocktail 只能保护蛋白的磷酸化状态防止去修饰,但是若想保证全蛋白的产出率,推荐使用Abmole 蛋白酶抑制剂Protease Cocktail。
附产品组成:
资料下载
Adv Sci (Weinh). 2023 Mar 23;e2207216.
Polydopamine Nanoparticles Targeting Ferroptosis Mitigate Intervertebral Disc Degeneration Via Reactive Oxygen Species Depletion, Iron Ions Chelation, and GPX4 Ubiquitination Suppression
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EMBO Rep. 2023 Mar 28;e55903.
PcEiger links the Imd/Relish pathway to ROS production in the intestine of the red swamp crayfish
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J Agric Food Chem. 2023 Apr 5;71(13):5185-5194.
Oral Delivery of Mouse β-Defensin 14 (mBD14)-Producing Lactococcus lactis NZ9000 Attenuates Experimental Colitis in Mice
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J Hypertens. 2023 Jan 13.
CircRNA_06354 might promote early-onset preeclampsia in humans via hsa-miR-92a-3p/vascular endothelial growth factor-A
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Protein Cell. 2022 Jan;13(1):47-64.
cGAS guards against chromosome end-to-end fusions during mitosis and facilitates replicative senescence
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Phytomedicine. 2022 Aug;103:154245.
Autophagy activation by Terminalia chebula Retz. reduce Aβ generation by shifting APP processing toward non-amyloidogenic pathway in APPswe transgenic SH
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Int Immunopharmacol. 2022 Sep 13;112:109133.
Inhibition of glutaminolysis ameliorates lupus by regulating T and B cell subsets and downregulating the mTOR/P70S6K/4EBP1 and NLRP3/caspase-1/IL-1β pathways in MRL/lpr mice
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
J Periodontal Res. 2022 Oct;57(5):1034-1042.
Impact of Resolvin D1 on the inflammatory phenotype of periodontal ligament cell response to hypoxia
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Iran J Basic Med Sci . 2022 May;25(5):635-642.
Transient receptor potential vanilloid type-1 regulates periodontal disease damage via the PI3K/AKT signaling pathway
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Poult Sci. 2022 Dec 8;102(2):102415.
Effect of Angelica Sinensis extract on the angiogenesis of preovulatory follicles (F1–F3) in late-phase laying hens
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
J Mater Chem B. 2021 May 12;9(18):3838-3855.
A hybrid membrane coating nanodrug system against gastric cancer via the VEGFR2/STAT3 signaling pathway
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
PLoS Genet. 2021 Apr 26;17(4):e1009514.
Subunit P60 of phosphatidylinositol 3-kinase promotes cell proliferation or apoptosis depending on its phosphorylation status
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Phytomedicine. 2021 Jul 15;88:153609.
Schisantherin A ameliorates liver fibrosis through TGF-β1mediated activation of TAK1/MAPK and NF-κB pathways in vitro and in vivo
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
J Proteome Res. 2021 Jan 1;20(1):357-368.
Polyunsaturated Fatty Acid Diet and Upregulation of Lipoxin A4 Reduce the Inflammatory Response of Preeclampsia
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Cell Death Dis. 2020 Oct 23;11(10):902.
The miR-30a-5p/CLCF1 axis regulates sorafenib resistance and aerobic glycolysis in hepatocellular carcinoma
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Front Cell Dev Biol. 2020 Dec 17;8:607757.
Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Front Oncol. 2020 May 21;10:667.
Downregulation of TET1 Promotes Bladder Cancer Cell Proliferation and Invasion by Reducing DNA Hydroxymethylation of AJAP1
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Phytochem Lett. 2020 Apr;36:17-23.
Artemargyinolide E, a new sesquiterpene lactone from Artemisia argyi inhibits inflammatory responses via down-regulating NF-κB signaling pathway
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Int J Mol Med. 2019 Jun;43(6):2440-2450.
Role of the thioredoxin interacting protein in diabetic nephropathy and the mechanism of regulating NOD‑like receptor protein 3 inflammatory corpuscle.
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Mol Med Rep. 2019 May;19(5):3658-3666.
Knockdown of serpin peptidase inhibitor clade C member 1 inhibits the growth of nasopharyngeal carcinoma cells.
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Mol Med Rep. 2019 May;19(5):3707-3713.
1,25‑Dihydroxy‑Vitamin D3 induces macrophage polarization to M2 by upregulating T‑cell Ig‑mucin‑3 expression.
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Int J Ophthalmol. 2019 Oct 18;12(10):1539-1547.
Inhibition of Obtusifolin on retinal pigment epithelial cell growth under hypoxia.
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Med Sci Monit. 2018 Dec 15;24:9110-9119.
Myosin Heavy Chain 10 (MYH10) Gene Silencing Reduces Cell Migration and Invasion in the Glioma Cell Lines U251, T98G, and SHG44 by Inhibiting the Wnt/β-Catenin Pathway.
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
Med Sci Monit. 2018 Nov 20;24:8348-8356.
Leucine-Rich Alpha-2-Glycoprotein1 Gene Interferes with Regulation of Apoptosis in Leukemia KASUMI-1 Cells.
Phosphatase Inhibitor Cocktail (EDTA-Free)购买自AbMole
产品仅供科学研究或药证申报的用途使用,不为任何个人或者非科研性质的其他用途提供服务。
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