蛋白酶抑制剂Cocktail（不含EDTA，100X DMSO储液）别名：Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)

目录号 M5293

AbMole蛋白酶抑制剂Cocktail（不含EDTA，100X DMSO储液）是一种低毒，全面的蛋白保护试剂，最大限度地保护蛋白免受蛋白酶降解，提高蛋白得率。本品可用于蛋白免疫印迹 (WB)、免疫共沉淀 (Co-IP)、pull-down、免疫沉淀 (IP)、免疫组织化学 (IHC)、免疫荧光 (IF)、酶学研究等多种与蛋白相关的实验。

Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)结构式

规格	价格	库存状态
1 mL	¥ 200	现货
1 mL x 10	¥ 1500	现货

*AbMole所有产品仅供有资质的科研机构或医药企业进行科学研究或药证申报用途，不能被用于人体和任何其它用途。我们不向任何个人或非科研性质的机构提供产品和服务。

客户使用AbMole的Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)发表的文献

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产品介绍

产品优势：

组分清晰：6种抑制剂按照固定浓度配比。
稳定高效：保留蛋白质的完整性，产出率较高。
物美价廉：质量保障同时，价格合理，为您节省研究开支。

实验数据：

产品描述：

本产品是由 6 种独立的蛋白酶抑制剂（不含 EDTA）： AEBSF、Aprotinin、 Leupeptin 、Bestatin、E-64、Pepstatin A 按照优化的配比配置而成的蛋白酶抑制剂混合物。其作用的靶点分别为丝氨酸蛋白酶，丝氨酸半胱氨酸蛋白酶，氨肽酶，半胱氨酸蛋白酶和天冬氨酸蛋白酶等，广泛作用于各种蛋白酶，可称之为广谱蛋白酶抑制剂。

使用方法：

1. 本产品适用于哺乳动物细胞及组织的蛋白质提取及纯化，蛋白免疫印迹（Western Blot）,免疫共沉(Co-IP),免疫荧光（IF） ,免疫组织化学（IHC）,激酶测定（kinase assay）和抗体、酶诊断试剂盒（DigKit）等。
2. 实验时，按照 1:100 的容积比，将 Cocktail 预先加入在已准备好的实验体系中，轻轻混合均匀。

保存条件：

本产品应于-20℃环境下密封保存，产品有效期为 2 年。使用时可短时间放置于常温或 4℃中，用毕应立即放回-20℃中保存。

注意事项：

1. 本产品不含 EDTA, 为 100× DMSO 储存液形式。使用时应调整 Cocktail 浓度为 1×，若有蛋白酶含量丰富的细胞或组织，可适当增加 Cocktail 在实验体系中的浓度。
2. 本产品置于-20℃保存时，DMSO 呈现冰晶状态，此属于正常现象，非产品质量问题。
3. 本产品仅供科研使用。

常见问题解答：

1. 蛋白酶抑制剂Cocktail有哪些优势？
回答：针对多种氨基酸的靶点，对目的蛋白进行全方位的保护，使其免受内源性蛋白酶的降解，蛋白的得出率较高，大大提高实验效率。
2. 可以配合细胞裂解液一起使用吗？
回答：可以，这样可以充分的裂解细胞或组织中的蛋白，有效降低蛋白质的降解。
3. 为什么需要用DMSO溶解？可否换成其他溶剂？
回答：蛋白酶抑制剂Cocktail较易溶解于DMSO，且DMSO通常不会影响实验结果，而且性质稳定，利于保存和运输。因此暂不考虑其他溶剂。
4. 实验时误将其放置常温下，会不会造成试剂变质？
回答：短时间放置于常温下是可以的，不会造成试剂变质，因为蛋白酶抑制剂通常比较稳定。如果实验需要在常温下进行，建议将其置于冰块操作，用毕后迅速放置-20℃保存。同时应尽量避免反复冻融。

资料下载

蛋白酶抑制剂Cocktail（不含EDTA，100X DMSO储液）使用说明书

实验参考

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。

动物实验方案计算器

请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。

1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。

延伸阅读（仅做信息扩展，不作实验参考）

1. 为什么蛋白质降解会成为WB实验的“绊脚石”？

在细胞裂解或组织提取过程中，原本隐藏在各种细胞器中的蛋白酶可能会被激活，从而导致蛋白质降解。这种降解不仅会破坏目标蛋白的完整性，还会影响后续的检测效果。例如，目标蛋白可能因为降解而无法被抗体特异性识别，或者在电泳过程中出现多条降解条带，干扰实验结果的解读。因此，从样本处理的第一步开始，防止蛋白质降解就显得至关重要。

2. AbMole蛋白酶抑制剂Cocktail（Protease Inhibitor Cocktail，M5293）：全方位守护蛋白完整性

AbMole的蛋白酶抑制剂Cocktail（M5293）是一款含有多款抑制剂的复合物，它包含了多种高效、特异性的蛋白酶抑制剂，由AEBSF、Aprotinin、Leupeptin、Bestatin、E-64、Pepstatin-A等6种不同的蛋白酶抑制剂优化配比配置而成，相较于传统的由单一组分构成的蛋白酶抑制剂如PMSF，能够更加广泛地抑制丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶等多种常见蛋白酶的活性。这种全方位的保护机制，从样本提取的那一刻起，就为您的目标蛋白提供了坚实的防护。另外一方面，PMSF具有一定的毒性和腐蚀性且半衰期较短。而AbMole的蛋白酶抑制剂Cocktai（M5293）则具有低毒和较强的稳定性，可有效解决大家使用PMSF时的担忧。AbMole的蛋白酶抑制剂Cocktai（Protease Inhibitor Cocktail，M5293）除了用于WB实验以外，还能为免疫共沉淀 (Co-IP)、pull-down、免疫沉淀 (IP)、免疫组织化学 (IHC)、免疫荧光 (IF)、酶学研究等多种实验中的蛋白提供保护。

图1. AbMole的蛋白酶抑制剂Cocktail（M5293）可有效保护目的蛋白不受蛋白酶的降解。

3. 范例详解

案例1. J Exp Clin Cancer Res. 2023 Jun 28;42(1):155

研究人员利用WB实验、代谢组学分析等技术筛选与PDAC肝转移有关的关键代谢物。实验中使用了来自AbMole的两个爆款产品，即蛋白酶抑制剂Cocktai （M5293）和磷酸酶抑制剂Cocktail（M7528）对蛋白进行保护，两款抑制剂有效避免了蛋白的降解并保护蛋白的磷酸化状态。

图2. 使用来自AbMole的蛋白酶抑制剂Cocktail（M5293）为WB实验提供保护^[1]

案例2. Advanced science 12(12) (2025) e2411719

研究人员发现FDFT1在肝细胞癌（HCC）中的表达与胆固醇和胆汁酸水平的变化相关，并通过HNF4A/ALDOB/AKT1轴影响HCC的进展。在研究FDFT1的功能时，实验人员使用来自AbMole的蛋白酶抑制剂Cocktail（M5293）为动物组织的蛋白提取提供保护。

图3. AbMole的蛋白酶抑制剂Cocktail（M5293）（为动物组织WB实验中的蛋白提供保护^[2]。

案例3. Cell death & disease 11(10) (2020) 902

科研人员研究了miR-30a-5p/CLCF1轴在肝细胞癌（HCC）中的Sorafenib耐受性和有氧糖酵解中的作用。在验证CLCF1的敲低以及对下游信号蛋白的影响时，实验人员使用了AbMole的蛋白酶抑制剂Cocktail（Protease Inhibitor Cocktail，M5293）为蛋白提供保护。

图4. 由AbMole提供的蛋白酶抑制剂Cocktail（M5293）为WB实验提供保护，以验证CLCF1的敲低^[3]

参考文献及鸣谢

[1] J. Yang, B. Ren, J. Ren, et al., Epigenetic reprogramming-induced guanidinoacetic acid synthesis promotes pancreatic cancer metastasis and transcription-activating histone modifications, Journal of experimental & clinical cancer research : CR 42(1) (2023) 155.

[2] D. Cai, G. C. Zhong, X. Dai, et al., Targeting FDFT1 Reduces Cholesterol and Bile Acid Production and Delays Hepatocellular Carcinoma Progression Through the HNF4A/ALDOB/AKT1 Axis, Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12(12) (2025) e2411719.

[3] Z. Zhang, X. Tan, J. Luo, et al., The miR-30a-5p/CLCF1 axis regulates sorafenib resistance and aerobic glycolysis in hepatocellular carcinoma, Cell death & disease 11(10) (2020) 902.