产品优势:
组分清晰:6种抑制剂按照固定浓度配比。
稳定高效:保留蛋白质的完整性,产出率较高。
物美价廉:质量保障同时,价格合理,为您节省研究开支。
实验数据:
产品描述:
本产品是由 6 种独立的蛋白酶抑制剂(不含 EDTA): AEBSF、Aprotinin、 Leupeptin 、Bestatin、E-64、Pepstatin A 按照优化的配比配置而成的蛋白酶抑制剂混合物。其作用的靶点分别为丝氨酸蛋白酶,丝氨酸半胱氨酸蛋白酶,氨肽酶,半胱氨酸蛋白酶和天冬氨酸蛋白酶等,广泛作用于各种蛋白酶,可称之为广谱蛋白酶抑制剂。
使用方法:
1. 本产品适用于哺乳动物细胞及组织的蛋白质提取及纯化,蛋白免疫印迹(Western Blot),免疫共沉(Co-IP),免疫荧光(IF) ,免疫组织化学(IHC),激酶测定(kinase assay)和抗体、酶诊断试剂盒(DigKit)等。
2. 实验时,按照 1:100 的容积比,将 Cocktail 预先加入在已准备好的实验体系中, 轻轻混合均匀。
保存条件:
本产品应于-20℃环境下密封保存,产品有效期为 2 年。使用时可短时间放置于常温或 4℃中,用毕应立即放回-20℃中保存。
注意事项:
1. 本产品不含 EDTA, 为 100× DMSO 储存液形式。使用时应调整 Cocktail 浓度为 1×,若有蛋白酶含量丰富的细胞或组织,可适当增加 Cocktail 在实验体系中的浓度。
2. 本产品置于-20℃保存时,DMSO 呈现冰晶状态,此属于正常现象,非产品质量问题。
3. 本产品仅供科研使用。
常见问题解答:
1. 蛋白酶抑制剂Cocktail有哪些优势?
回答:针对多种氨基酸的靶点,对目的蛋白进行全方位的保护,使其免受内源性蛋白酶的降解,蛋白的得出率较高,大大提高实验效率。
2. 可以配合细胞裂解液一起使用吗?
回答:可以,这样可以充分的裂解细胞或组织中的蛋白,有效降低蛋白质的降解。
3. 为什么需要用DMSO溶解?可否换成其他溶剂?
回答:蛋白酶抑制剂Cocktail较易溶解于DMSO,且DMSO通常不会影响实验结果,而且性质稳定,利于保存和运输。因此暂不考虑其他溶剂。
4. 实验时误将其放置常温下,会不会造成试剂变质?
回答:短时间放置于常温下是可以的,不会造成试剂变质,因为蛋白酶抑制剂通常比较稳定。如果实验需要在常温下进行,建议将其置于冰块操作,用毕后迅速放置-20℃保存。同时应尽量避免反复冻融。
资料下载
蛋白酶抑制剂Cocktail(不含EDTA,100X DMSO储液)使用说明书
Adv Sci (Weinh). 2023 Mar 23;e2207216.
Polydopamine Nanoparticles Targeting Ferroptosis Mitigate Intervertebral Disc Degeneration Via Reactive Oxygen Species Depletion, Iron Ions Chelation, and GPX4 Ubiquitination Suppression
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Protein Cell. 2022 Jan;13(1):47-64.
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Acta Pharmacol Sin. 2022 Oct 7;Online ahead of print..
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Phytomedicine. 2022 Aug;103:154245.
Autophagy activation by Terminalia chebula Retz. reduce Aβ generation by shifting APP processing toward non-amyloidogenic pathway in APPswe transgenic SH
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J Anim Sci Biotechnol. 2022 Feb 8;13(1):27.
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Front Microbiol. 2022 Jun 9;13:927122.
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J Oncol. 2022 May 31;2022:3909030.
Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells
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Research Square. 2022 Feb.
Dexmedetomidine preconditioning alleviates H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells via p38MAPK signaling pathway
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BMC Biol. 2021 May 20;19(1):108.
Very long intergenic non-coding (vlinc) RNAs directly regulate multiple genes in cis and trans
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Mater Sci Eng C Mater Biol Appl. 2021 Dec;131:112503.
Superior low-immunogenicity of tilapia type I collagen based on unique secondary structure with single calcium binding motif over terrestrial mammals by inhibiting activation of DC intracellular Ca2+-mediated STIM1-Orai1/NF-кB pathway
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Aging Cell. 2021 Jan;20(1):e13282.
SPATA4 improves aging-induced metabolic dysfunction through promotion of preadipocyte differentiation and adipose tissue expansion
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PLoS Pathog. 2021 Feb 8;17(2):e1008992.
CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreatitis
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J Mater Chem B. 2021 May 12;9(18):3838-3855.
A hybrid membrane coating nanodrug system against gastric cancer via the VEGFR2/STAT3 signaling pathway
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Phytomedicine. 2021 Jul 15;88:153609.
Schisantherin A ameliorates liver fibrosis through TGF-β1mediated activation of TAK1/MAPK and NF-κB pathways in vitro and in vivo
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First insights into the autophagy machinery of adult Schistosoma mansoni
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PeerJ. 2021 Oct 26;9:e12312.
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