CCK试剂盒(Cell Counting Kit),为MTT 法的替代方法,是一种基于WST(水溶性四唑盐,化学名:2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)的广泛应用于细胞增殖和细胞毒性的快速高灵敏度检测试剂盒。
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Knockdown of MON1B Exerts Anti-Tumor Effects in Colon Cancer In Vitro.
CCK试剂盒简介
CCK试剂盒(Cell Counting Kit),为MTT 法的替代方法,是一种基于WST(水溶性四唑盐,化学名:2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)的广泛应用于细胞增殖和细胞毒性的快速高灵敏度检测试剂盒。
WST检测原理图
CCK法与其它检测方法之间的比较
CCK用途:药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验。
CCK试剂盒的优点及应用
CCK试剂盒使用方便,省去了洗涤细胞,不需要同位素和有机溶剂
CCK法的检测灵敏度很高,甚至可以测定较低细胞密度
CCK试剂盒为1瓶溶液,毋需预制,即开即用
CCK法的重复性优于MTT法
CCK试剂毒性
CCK和其他检测方法对比可以得出——CCK对细胞几乎无毒性,不伤害细胞,细胞可重复利用。
资料下载
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。
细胞活力检测实验方法
细胞活力检测是细胞生物学研究中的基础且关键环节,广泛应用于细胞增殖检测、细胞毒性评估、药物筛选等多个领域。传统的MTT法虽然应用广泛,但存在诸多不足:操作繁琐、灵敏度较低、因孔板倒扣时结晶损失导致的读值误差、且MTT法本身对细胞就有潜在毒性。CCK-8试剂盒的出现,有效解决了这些问题。然而,目前市场上大部分CCK-8试剂盒存在一些问题:如受到细菌污染导致读值偏差,长期储存容易变色进而影响检测结果的准确性。AbMole拥有 严格的品控体系,AbMole的CCK-8试剂盒有效避免了上述问题,并被国内外大量科研单位和制药公司使用。
图1. 使用AbMole CCK-8试剂盒的细胞活力检测结果
CCK-8检测原理
CCK-8试剂盒是一种基于WST-8的细胞增殖和毒性检测试剂盒。其核心成分WST-8在细胞线粒体中的脱氢酶作用下,可被还原为具有高度水溶性的橙黄色甲瓒产物(Formazan),生成的甲瓒物数量与活细胞数量成正比。这种检测方法无需洗涤细胞,也不需要使用有机溶剂,操作简便,减少了实验误差。2014年,AbMole的两款抑制剂分别被西班牙国家心血管研究中心和美国哥伦比亚大学用于动物体内实验,相关科研成果发表于顶刊 Nature 和 Nature Medicine。
图2.CCK-8的检测原理图
产品优势
AbMole的CCK-8试剂盒具有以下多种优势:
无污染:经过严格的无菌生产,不会出现细菌污染导致读值偏差,解决细胞活力检测实验中易被污染的“痛点”。
无挂壁:配制好的CCK-8工作液不会贴附在孔板壁上,减少误差,读值更准确。
长期储存不易变色:可在2-8℃的环境中长期储存,不会出现变色和读值衰减的情况。
灵敏度高:检测灵敏度高于传统的MTT法,即使在较低的细胞密度下也能准确检测。
操作简便:试剂为即用型溶液,无需预制,直接加入细胞培养基中即可。
数据可靠:检测结果与细胞数量呈线性关系,重复性好,适合高通量筛选。
产品引用
AbMole的CCK-8试剂盒被国内外大量课题组引用,相关文献发表于多个高水平期刊。选择AbMole的CCK-8试剂盒,为您的细胞活力检测实验提供可靠的保障!以下是使用AbMole的CCK-8发表的相关文献(因篇幅原因,仅为部分展示):
Adv Sci (Weinh). 2025 Feb 03.
Acta Biomater. 2025 Jan 26.
Int J Mol Med. 2025 Jan 17;55(3):47.
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Mol Cancer. 2024 Jan;23(1):27.
Adv Funct Mater. 2024 Dec 02.
Nat Commun. 2024 Mar 9;15(1):2163.
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Chem Eng J. 2024 Sep 18.
J Control Release. 2024 Aug 09.
Applied Materials Today. 2024 Jan;36.
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