Recombinant Human MMP12 Protein (E. coli) 别名：Matrix metalloproteinase-12; 重组人MMP12 蛋白 (E. coli)

目录号 M43971

Recombinant Human MMP12 Protein (E. coli)结构式

规格	价格	库存状态
20ug	¥ 4050	现货

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质量标准及产品资料

纯度：>90%, Endotoxin < 1 EU/µg
COA

化学性质/溶解性/储存

性状	Lyophilized powder
中文名称	重组人MMP12 蛋白 (E. coli)
储存条件	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

生物活性

Matrix metalloproteinase-12 (MMP12) is a macrophage-secreted elastase that is highly induced in the liver and lung in response to S. mansoni eggs and contains four hemopexin-like domains. MMP12 is a proteolytic enzyme responsible for the cleavage of plasminogen to angiotensin, which has an angiostatic effect. MMP12 promotes fibrosis by limiting the expression of specific ECM-degrading MMPs. MMP12 is a potent proinflammatory and oncogenic molecule. MMP12 up-regulation plays a critical role in emphysema to lung cancer transition that is facilitated by inflammation.

The recombinant human MMP12 consists of 164 amino acids and predicts a molecular mass of 18.2 KDa. A DNA sequence encoding human MMP12 (Gly106-Asn268) was expressed.

Measured by its ability to cleave the fluorogenic peptide substrate (Mca-PLGL-Dpa-AR-NH2), the specific activity is > 800 pmoles/min/µg.

Endotoxin < 1 EU/µg

Apparent Molecular Weight: 18 KDa, reducing conditions

Lyophilized from sterile 10 mM Hepes, 2 mM CaCl2, 250 mM NaCl, pH 7.0.

实验参考

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。