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Recombinant Schistosoma Japonicum GST (E.coli)

目录号 M21326 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


重组日本血吸虫谷胱甘肽S-转移酶Class-mu 26 kDa Isozyme由大肠杆菌表达系统生产,编码Met1-Lys218的目标基因被表达。蛋白编号: P08515。

Recombinant Schistosoma Japonicum GST (E.coli)结构式

别名:Glutathione S-transferase class-mu 26 kDa isozyme; GST 26

规格 价格 库存状态
10ug ¥ 900 中国库存现货
50ug ¥ 1305 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >95% , Endotoxin < 1 EU/µg
  • COA
  • MSDS
生物活性

表达系统:E.coli

表观分子量:28 KDa,还原条件

纯度:通过还原SDS-PAGE测定,大于95%

内毒素:经LAL测试,小于0.1 ng/µg(1 EU/µg)

存储及稳定性:冻干的蛋白质应储存在<-20°C,尽管在室温下可稳定3周。重组的蛋白质溶液可在4-7°C储存2-7天。重组的样品等分在<-20°C下可稳定3个月。


生物活性:谷胱甘肽S-转移酶(GSTs),以前被称为配体蛋白,包括真核和原核第二阶段代谢同工酶家族,最有名的是它们能够催化谷胱甘肽(GSH)的还原形式与异生物底物的共轭以达到解毒的目的。GST家族由三个超级家族组成:细胞膜、线粒体和微粒体(MAPEG)蛋白。GST同工酶似乎在寄生虫的解毒系统中起着核心作用。其他功能也被怀疑包括在增加寄生虫肠道中血色素的溶解度方面的作用。GSTs的活性取决于来自合成酶γ-谷氨酰半胱氨酸合成酶和谷胱甘肽合成酶的GSH的稳定供应,以及特定转运器的作用,将GSH的共轭物从细胞中移除。GSTs的主要作用是通过催化GSH对所述非极性异生物底物的亲电碳、硫或氮原子的亲核攻击来解毒异生物,从而防止它们与关键的细胞蛋白和核酸相互作用。

化学性质
溶解性(25°C) It is not recommended to reconstitute to a concentration less than 100μg/ml.
储存条件 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] You Li, et al. Vet Parasitol. Establishment and evaluation of an iELISA using the recombinant membrane protein LHD-Sj23 for the serodiagnosis of Schistosoma japonicum infection in cattle in China

[2] Z Zhu, et al. Parasite Immunol. Protective efficacy evaluation induced by recombinant protein LHD-Sj23-GST of Schistosoma japonicum emulsified with three different adjuvants

[3] Man-jun Li, et al. Exp Parasitol. Cimetidine enhances the protective effect of GST DNA vaccine against Schistosoma japonicum

[4] Guangqing Yu, et al. J Huazhong Univ Sci Technolog Med Sci. Expression of recombinant baculovirus carrying Schistosoma japonicum 26 ku GST in mammalian cells

[5] Nobuo Ohta, et al. Parasitol Int. Research on calpain of Schistosoma japonicum as a vaccine candidate

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