Recombinant Rat EGF Protein (CHO) 别名：Epidermal Growth Factor; 重组大鼠EGF蛋白 (CHO)
Recombinant Rat EGF Protein is type Itransmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc.
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组大鼠EGF蛋白 (CHO) |
| 溶解性 | Reconstitute the lyophilized powder in ddH2O to 100 μg/ml. |
| 储存条件 | Stable for up to 6 months at ≤ -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Recombinant Rat EGF Protein is type Itransmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Measured in a cell proliferation assay using 3T3 cells, the ED50 is < 0.1 ng/ml, and the corresponding specific activity is ≥ 1 x 107 IU/mg.
Accession: P07522
Endotoxin < 0.2 EU/μg
Apparent Molecular Weight: ~6 kDa, reducing conditions
Lyophilized after extensive dialysis against PBS.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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