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Recombinant Rat CNTF (E. coli) 重组大鼠CNTF蛋白 (E. coli)

目录号 M15020 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


重组大鼠CNTF蛋白 (E. coli)是一种作用于神经系统的多肽激素,在某些神经元群体中促进神经递质合成和神经突生长。蛋白构造:CNTF (Ala2-Met200),Accession # P20294-1。

Recombinant Rat CNTF (E. coli)结构式

别名:Ciliary Neurotrophic Factor

规格 价格 库存状态
10ug ¥ 1395 中国库存现货
50ug ¥ 3465 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
生物活性

种属:Rat

纯度:SDS-PAGE分析,> 95%

内毒素:凝胶凝血法测定蛋白质< 0.2 EU/μg

表达系统:E. coli

表观分子量:~22.9 kDa,还原条件下在SDS-PAGE上

存储及稳定性:在接收后,该产品在低于-70°C的环境下保持稳定长达6个月。重组后,产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存,建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环


生物活性:ED50 < 30.0 ng/ml,通过诱导TF-1细胞产生碱性磷酸酶的能力。CNTF是神经元和少突胶质细胞的一个有效的生存因子,可能在炎症增加时起到减少组织损伤的作用。该基因的突变导致异常剪接,导致睫状神经营养因子缺乏,但这种表型与神经疾病没有因果关系。

化学性质
溶解性(25°C) Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.
储存条件 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] Longfu Xu, et al. Protein Expr Purif. Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain

[2] Isabelle Cognet, et al. Eur Cytokine Netw. Expression of biologically active mouse ciliary neutrophic factor (CNTF) and soluble CNTFRalpha in Escherichia coli and characterization of their functional specificities

[3] I Saggio, et al. Anal Biochem. Nonradioactive receptor binding assay for ciliary neurotrophic factor

[4] K Ohta, et al. Biochem Mol Biol Int. Tissue expression of rat ciliary neurotrophic factor (CNTF) mRNA and production of the recombinant CNTF

[5] M Thier, et al. J Neurosci Res. Site-directed mutagenesis of human CNTF: functional analysis of recombinant variants

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