重组大鼠CNTF蛋白 (E. coli)是一种作用于神经系统的多肽激素,在某些神经元群体中促进神经递质合成和神经突生长。蛋白构造:CNTF (Ala2-Met200),Accession # P20294-1。
别名:Ciliary Neurotrophic Factor
种属:Rat
纯度:SDS-PAGE分析,> 95%
内毒素:凝胶凝血法测定蛋白质< 0.2 EU/μg
表达系统:E. coli
表观分子量:~22.9 kDa,还原条件下在SDS-PAGE上
存储及稳定性:在接收后,该产品在低于-70°C的环境下保持稳定长达6个月。重组后,产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存,建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环
生物活性:ED50 < 30.0 ng/ml,通过诱导TF-1细胞产生碱性磷酸酶的能力。CNTF是神经元和少突胶质细胞的一个有效的生存因子,可能在炎症增加时起到减少组织损伤的作用。该基因的突变导致异常剪接,导致睫状神经营养因子缺乏,但这种表型与神经疾病没有因果关系。
溶解性(25°C) | Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
储存条件 |
粉末型式 -20°C 3年;4°C 2年 溶于溶剂 -80°C 6个月;-20°C 1个月 |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
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