Recombinant Mouse/Rat VEGF-C Protein (HEK293, aa 108-223, His Tag) 别名：Vascular endothelial growth factor C; 重组小鼠/大鼠VEGF-C蛋白 (HEK293, aa 108-223, His Tag)
Recombinant Mouse/Rat VEGF-C Protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC), the ED50 for this effect is typically 0.1-0.8 μg/mL.
化学性质/溶解性/储存
| 中文名称 | 重组小鼠/大鼠VEGF-C蛋白 (HEK293, aa 108-223, His Tag) |
| 溶解性 | Reconstitute in ddH2O or PBS at 100 μg/mL |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Recombinant Mouse/Rat VEGF-C Protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis.
Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC), the ED50 for this effect is typically 0.1-0.8 μg/mL.
A DNA sequence encoding the mouse/rat VEGFC (NP_033532.1/O35757) (Ala108-Arg223) was expressed fused with a polyhistidine tag at the C-terminus.
The recombinant mouse/rat VEGFC comprises 127 amino acids and predicts a molecular mass of 14.5 kDa.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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