ED50 < 0.05 ng/ml,通过使用M-NFS-60细胞的细胞增殖试验测量,对应的特异性活性> 2.0 × 10 7单位/毫克。Accession # P01586。
别名:IL3; interleukin 3; IL-3; MCGF
种属:Mouse
表达系统:E. coli
纯度:通过SDS-PAGE分析,>95%
内毒素:通过凝胶凝固法,< 0.2 EU/μg的蛋白质
表观分子量:~15.2 kDa,在非还原条件下的SDS-PAGE上
存储及稳定性:本品收到后,在低于-70°C的环境下可保持长达6个月的稳定性。重组后,本品在4°C下应可稳定1周,或在-20°C下可稳定3个月。对于长期储存,建议加入载体蛋白(如0.1% BSA)。避免重复冷冻-解冻循环。
生物活性:白细胞介素-3(IL-3)是属于白细胞介素家族的一种多态性细胞因子。IL-3与粒细胞-巨噬细胞集落刺激因子(GM-CSF)和IL-5有相似之处:它们都有一个四螺旋束结构,在人类和小鼠中都位于相同的染色体上,由活化的T细胞产生,并且有共同的受体。IL-3/IL-5/GM-CSF受体家族成员都是异源二聚体,由一条受体特异性α链和一条共同的β链组成。IL-3也被称为多集落刺激因子,因为它通过激活细胞内的Ras-Raf-ERK和JAK/STAT等途径刺激多系造血细胞的发育和集落形成。IL-3通过靶向抗凋亡的bcl-2基因家族抑制细胞凋亡并促进细胞生存。
溶解性(25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
储存条件 |
粉末型式 -20°C 3年;4°C 2年 溶于溶剂 -80°C 6个月;-20°C 1个月 |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
[4] A Kusumi, et al. Gan To Kagaku Ryoho. [Interleukin 3 (IL-3)]
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