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Recombinant Mouse FGF-9 (E. coli) 重组小鼠FGF-9 蛋白(E. coli)

目录号 M15031 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


成纤维细胞生长因子-9 (FGF-9)是一种多效细胞因子,属于肝素结合FGF家族。像家族中的其他成员一样,FGF-9类似于β-三叶结构。蛋白构造:Expressed with an N-terminal Met. FGF-9 [Pro3-Ser208(Ser34Asn)],Accession # P54130。

Recombinant Mouse FGF-9 (E. coli)结构式

别名:Fibroblast Growth Factor-9; FGF9

规格 价格 库存状态
10ug ¥ 1845 中国库存现货
50ug ¥ 4095 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >95% , Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
生物活性

种属:Mouse

表达系统:E. coli

纯度:SDS-PAGE分析,> 95%,高效液相色谱分析> 95%
内毒素:凝胶凝血法测定蛋白质< 0.2 EU/μg

表观分子量:~23.4 kDa,还原条件下在SDS-PAGE上

存储及稳定性:在接收后,该产品在低于-70°C的环境下保持稳定长达6个月。重组后,产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存,建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环。


生物活性:ED50 < 5.0 ng/ml,采用3T3细胞增殖法测定,对应的比活性为> 2.0 × 105单位/mg。FGF-9发生可逆二聚,这是其亚家族成员FGF-16和FGF-20的共同特征。同源二聚化过程中涉及的突变也影响肝素的亲和力、与FGF受体的结合以及生物活性。在体内,FGF-9在肢体芽、发育中的骨骼和胚胎发生后期的肠道中表达。FGF-9对心脏、肺、肾、盲肠和睾丸的发育至关重要;FGF-9水平的降低导致早分化。FGF-9还与骨形态发生蛋白7 (BMP-7)一起促进肾单位祖细胞的存活。

化学性质
溶解性(25°C) It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml.
储存条件 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] Shen Wang, et al. Appl Microbiol Biotechnol. Expression and purification of an FGF9 fusion protein in E. coli, and the effects of the FGF9 subfamily on human hepatocellular carcinoma cell proliferation and migration

[2] S Santos-Ocampo, et al. J Biol Chem. Expression and biological activity of mouse fibroblast growth factor-9

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