Recombinant Human Wnt3a Protein (HEK293) 别名：Wnt Surrogate-Fc Fusion Protein; 重组人Wnt3a 蛋白 (HEK293)
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family. WNT signaling plays key roles in the control of development, homeostasis, and regeneration of many essential organs and tissues.
化学性质/溶解性/储存
| 性状 | Solution |
| 中文名称 | 重组人Wnt3a 蛋白 (HEK293) |
| 储存条件 | Store at ≤ -70°C, stable for 6 months after receipt. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family. WNT signaling plays key roles in the control of development, homeostasis, and regeneration of many essential organs and tissues. Endogenous WNTs bind to multiple FZDs and are heavily modified posttranslationally, making them difficult to manufacture consistently. WNT surrogate molecules phenocopied endogenous ligand activities in in vitro reporter assays and intestine organoid functional assays.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells, the ED50 for this effect is 71.45 ng/ml.
Endotoxin < 0.01 EU/µg
Apparent Molecular Weight: 66-73kDa, reducing conditions
Supplied as a 0.2 μm filtered solution of 25mM Tris-HCl, 500mM NaCl, pH8.2.
The product is shipped on dry ice. Upon receipt, store it immediately at the temperature listed below.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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