Recombinant Human TNF RII (HEK293, C-6His) 别名：Tumor Necrosis Factor Receptor II; TNFRSF1B; 重组人TNF RII (HEK293, C-6His)
Recombinant Human TNF RII belongs to the TNFR (tumor necrosis factor receptor) superfamily characterized by cysteine-rich extracellular domains. TNFRII is strongly expressed at the cartilage-pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA).
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人TNF RII (HEK293, C-6His) |
| 溶解性 | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Recombinant Human TNF RII belongs to the TNFR (tumor necrosis factor receptor) superfamily characterized by cysteine-rich extracellular domains. TNFRII is strongly expressed at the cartilage-pannus junction, and plays a major role in a subset of families with multiple cases of rheumatoid arthritis (RA). This receptor mediates most of the metabolic effects of TNF-alpha function by antagonizing its biological activity.
Recombinant Human Tumor Necrosis Factor Receptor II expressed the target gene encoding Leu23-Asp257 with a 6His tag at the C-terminus.
The ED50 of Recombinant Human TNF RII (C-6His) is 0.212 ug/ml.
Accession: P20333
Endotoxin < 1 EU/µg
Apparent Molecular Weight: 40 KDa, reducing conditions
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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参考文献
