Recombinant Human SDF-1 Protein (E. coli) 别名：Stromal cell-derived factor-1; 重组人SDF-1蛋白 (E. coli)
The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF-1 binds primarily to CXC receptor 4 (CXCR4; CD184). The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription.
化学性质/溶解性/储存
| 中文名称 | 重组人SDF-1蛋白 (E. coli) |
| 溶解性 | Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml. |
| 储存条件 | Stable for up to twelve months from date of receipt at -20℃ to -80℃ |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
The human stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF-1 binds primarily to CXC receptor 4 (CXCR4; CD184). The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. SDF-1/CXCL12 has crucial roles in the formation of multiple organ systems during embryogenesis and in the regulation of bone marrow haematopoiesis and immune function in the postnatal organism.
Recombinant Human SDF-1 is produced by E.coli expression system and the target gene encoding Lys 22-Met 93 is expressed.
The recombinant human SDF-1 consists of 73 amino acids and predicts a molecular mass of 8.6 kDa. It migrates as an approximately 12 kDa band in SDS-PAGE under reducing conditions.
Accession: P48061
Endotoxin: < 1.0 EU per μg protein as determined by LAL test
Lyophilized from sterile PBS, pH 7.4.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
其他相关的Cytokines and Growth Factors产品
参考文献
