Recombinant Human RBP4 (HEK293, C-His) 别名：Retinol-Binding Protein 4; 重组人RBP4 (HEK293, C-His)
Human-retinol binding protein is a single-chain polypeptide with a molecular weight of approximately 21000 and one binding site for retinol and other forms of vitamin A. In plasma, RBP4-retinol forms a complex with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision.
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人RBP4 (HEK293, C-His) |
| 溶解性 | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| 储存条件 | Stable for up to 6 months at ≤ -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Human-retinol binding protein is a single-chain polypeptide with a molecular weight of approximately 21000 and one binding site for retinol and other forms of vitamin A. In plasma, RBP4-retinol forms a complex with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision.
Measured by its ability to bind all-trans retinoic acid, the binding of retinoic acid results in the quenching of Trp fluorescence in RBP4 > 1.0 µM all-trans retinoic acid is bound under the described conditions.
Accession: P02753
Apparent Molecular Weight: ~22 kDa, reducing conditions
Endotoxin < 0.2 EU/μg
Lyophilized from a 0.2 μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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