The recombinant Human PPARA consists of 276 amino acids and predicts a molecular mass of 31.36 kDa. A DNA sequence encoding the Human PPARA (Q07869) (Thr200-Tyr468) was expressed, with a polyhistidine tag at the N-terminus.
中文名称 | 重组人PPARA蛋白 (E.coli, His Tag) |
储存条件 | Stable for up to 6 months from date of receipt at ≤ -20°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
The recombinant Human PPARA consists of 276 amino acids and predicts a molecular mass of 31.36 kDa. A DNA sequence encoding the Human PPARA (Q07869) (Thr200-Tyr468) was expressed, with a polyhistidine tag at the N-terminus. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor.
Endotoxin <1 EU/µg
Lyophilized from sterile PBS, 10% Glycerol, 0.5% SKL, pH 8.0.
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。