Recombinant Human PD-L1 Protein (HEK293, C-Fc) 别名：Programmed Cell Death 1 Ligand 1; B7 Homolog 1; 重组人PD-L1 蛋白 (HEK293, C-Fc)
PD-L1 (also known as CD274, B7-H1) is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人PD-L1 蛋白 (HEK293, C-Fc)；程序性死亡配体 1 |
| 溶解性 | Reconstitute the lyophilized powder in distilled water to a concentration not less than 100 μg/mL. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
PD-L1 (also known as CD274, B7-H1) is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Recombinant Human PD-L1 expressed the target gene encoding Phe19-Thr239 with a human IgG1 Fc tag at the C-terminus.
Immobilized Human PD-L1 at 2μg/ml (100 μl/well) can bind Anti-Human PDL1 mAb-Fc, the ED50 of Anti-Human PDL1 mAb-Fc is 8.41 ng/ml.
Accession: Q9NZQ7
Apparent Molecular Weight: 70-80 KDa, under reducing conditions
Endotoxin < 1 EU/µg
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
其他相关的Immune Checkpoint Proteins产品
