Recombinant Human MFGE8 Protein (Baculovirus-Insect, His-AVI, Biotinylated) 别名：MFG-E8; milk fat globule-EGF factor 8 protein; 重组人MFGE8蛋白 (Baculovirus-Insect, His-AVI, Biotinylated)
MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell.
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人MFGE8蛋白 (Baculovirus-Insect, His-AVI, Biotinylated) |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to the phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. MFG-E8 has opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell.
The recombinant human MFGE8 consists of 390 amino acids and predicts a molecular mass of 44.1 kDa.
Biotin/Protein Ratio: 0.7-1 as determined by the HABA assay
Endotoxin < 1.0 EU/μg
Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 8.5, 10 % glycerol, 0.2 M Arginine, 0.01 % Tween-20.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
其他相关的Other Proteins产品
参考文献
