Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain. LGALS3 (Galectin 3) has an important role in tumor progression through inhibition of apoptosis.
性状 | Lyophilized powder |
中文名称 | 重组人Galectin-3 蛋白 (HEK293, C-His) |
溶解性 | Reconstitute the lyophilized powder in distilled water to a concentration not less than 100 μg/mL. |
储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain. LGALS3 (Galectin 3) has an important role in tumor progression through inhibition of apoptosis.
The recombinant human Galectin-3 consists of 260 amino acids and has a calculated molecular mass of 27.5 KDa. A DNA sequence encoding the human Galectin-3 (Ala2-Ile250) was expressed with a polyhistidine tag at the C-terminus.
Accession: P17931
Apparent Molecular Weight: 35 kDa, under reducing conditions
Endotoxin < 1 EU/µg
Lyophilized from sterile PBS, 2 mM Tcep, pH 6.5.
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。