重组人4-1BB配体由大肠杆菌表达系统产生,编码Arg71-Glu254的目的基因在c端以6His标记表达。Accession #: P41273。
别名:Tumor necrosis factor ligand superfamily member 9;
种属:Human
表达系统:E.coli
纯度:还原SDS-PAGE测定大于95%
内毒素:经LAL测试,小于0.1 ng/ug (1 EU/ug)
存储及稳定性:冻干蛋白应在< -20°C保存,但在室温下可保存3周。重组蛋白溶液可在4-7℃下保存2-7天。重组样品的等分液在< -20°C下稳定3个月。
生物活性:肿瘤坏死因子配体超家族成员9(4-1BBL)是单通型II膜蛋白,是肿瘤坏死因子家族成员之一。4-1BBL是一种254种氨基酸的细胞因子,在大脑、胎盘、肺、骨骼肌和肾脏中表达。TNFSF9已被证明除了促进T淋巴细胞增殖外,还能重新激活无能T淋巴细胞。该细胞因子可能在激活诱导细胞死亡(AICD)和t细胞与b细胞/巨噬细胞之间的同源相互作用中发挥作用。它也被证明是CD8 T细胞中最佳CD8反应所必需的,并被认为与T细胞-肿瘤细胞相互作用有关。
溶解性(25°C) | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
储存条件 |
粉末型式 -20°C 3年;4°C 2年 溶于溶剂 -80°C 6个月;-20°C 1个月 |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
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