人MCP-1/CCL2对CHO-K1/Gα15/hCCR2(人Gα15和人CCR2在CHO-K1细胞中稳定表达)Ca2+动员实验的EC50值小于1.0 μg/ml。Accession # : P13500。
别名：MCAF; JE; Monocyte Chemotactic Protein-1; CCL2; MCP1
表达系统：E. coli
纯度：SDS-PAGE分析> 95%
表观分子量：~8.7 kDa，在SDS-PAGE还原条件下
内毒素：凝胶凝血法测定蛋白质< 0.2 EU/μg
存储及稳定性：在接收后，该产品在低于-70°C的环境下保持稳定长达6个月。重组后，产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存，建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环。
生物活性：CCL2（E. coli），也被称为单核细胞趋化和激活因子(MCAF)，最初由两组独立纯化，基于其趋化吸引单核细胞的能力。在其克隆和测序之后，很明显，该蛋白也与人类乙脑基因的产物相同。乙脑基因最初在小鼠成纤维细胞中发现，是一种血小板衍生生长因子(PDGF)诱导基因。人CCL2 cDNA编码一个99个氨基酸残基的前体蛋白和一个23个残基的疏水信号肽，剪切后生成76个残基的成熟蛋白。天然的CCL2由于加入了连接碳水化合物和唾液酸残基，在大小上是不均一的。除fibroblasts;肿瘤细胞外，平滑肌细胞、内皮细胞和单核吞噬细胞也可构成性地或在各种刺激的刺激下产生CCL2。CCL2是趋化因子β (CC)亚家族的成员。最近，有报道称存在与CCL2氨基酸同源性分别为62%和73%的MCP2和MCP3。
| 溶解性（25°C） | Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| 储存条件 |
粉末型式 -20°C 3年；4°C 2年 溶于溶剂 -80°C 6个月；-20°C 1个月 |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
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蛋白质研究