2,3-Butanedione monoxime  别名：Biacetyl monoxime; Diacetyl monoxime; DAM; 二乙酰一肟

2,3-Butanedione monoxime (BDM) chemically removes phosphate groups from many proteins, and its action on DRK1 channels was examined after expression of DRK1 cRNA in Xenopus oocytes. In two-microelectrode voltage-clamp experiments, the application of 2,3-Butanedione monoxime to the bath inhibited DRK1 current (ki = 16.6 mM, H = 0.96) rapidly and reversibly, with a time course similar to the time course of solution change within the bath. DRK1 current was inhibited at all potentials; the time course of current activation, deactivation and inactivation were unaffected by 2,3-Butanedione monoxime. In inside-out patch-clamp experiments, the application of 2,3-Butanedione monoxime to the cytoplasmic surface similarly inhibited channel activity rapidly and reversibly (ki = 10.7 mM, H = 1.01) in the absence of rephosphorylating substrates. These results are inconsistent with a phosphatase effect, because such an effect should be irreversible in cell-free, ATP-free patches. Instead, the results suggest that 2,3-Butanedione monoxime can inhibit DRK1 channels directly from inside or outside of the membrane.

近来，一种可以使心肌肌球蛋白与肌动蛋白解偶联的药物2，3-丁二酮单肟(2，3-butanedionemonoxime，BDM)作为心肌保护Chemicalbook的“第三种概念”，越来越引人注目。一些研究证实，BDM可以增加兔、鼠和人的心脏对冷缺血的耐受性，可促进L-型Ca2+通道失活，减少心肌能耗。