Triton X-100  别名：曲拉通

目录号 M9162

Triton X-100（曲拉通X-100）是一种常用的非离子表面活性剂，能溶于水、甲苯、二甲苯和乙醇，不溶于石油醚。

Triton X-100结构式

  CAS No.：9002-93-1

规格 价格 库存状态
Free Sample (0.5-1 mg)  ¥ 0 现货
50mL ¥ 200 现货
100mL ¥ 280 现货
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客户使用AbMole的Triton X-100发表的文献

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质量标准及产品资料
  • 纯度：for molecular biology
  • COA
化学性质/溶解性/储存
分子式 C14H22O.n(C2H4O)
CAS号 9002-93-1
中文名称 曲拉通；辛基苯基聚氧乙烯醚
溶解性（仅列举部分溶剂） Soluble in water
储存条件 room temperature
运输方式 冰袋运输，根据产品的不同，可能会有相应调整。

*不同实验中用到的溶剂可能不同，具体实验所需溶剂及溶解方法请参考相关文献描述。

生物活性

曲拉通X-100是一种常用的非离子表面活性剂，能溶于水、甲苯、二甲苯和乙醇，不溶于石油醚。Triton X-100用于裂解细胞以提取蛋白和细胞器。Triton X-100还可通透活细胞膜用于转染。曲拉通X-100能使亲水性的蛋白质从两亲性的蛋白质中溶解出来，能够对膜外周蛋白和膜整合蛋白进行亚组分分离，更具有灵活性。


使用方法参考：

1. Triton X-100 细胞裂解液
由Triton X-100、NaCl、Tris-HCl 等组成的 Triton X-100 细胞裂解液是一种经典的快速裂解细胞组织并获得蛋白的裂解液。所获得的蛋白质可以用于 PAGE 电泳，Western，免疫沉淀 IP 和免疫共沉淀 co-IP 等。作用原理是利用去污剂Triton X-100破坏脂质双分子层，溶解胞质和细胞膜，破坏分子间微弱结合键的大部分蛋白质抗原。其浓度在0.1%～1%时即可满足几乎所有溶解的需求。

2. 免疫细胞化学应用
免疫细胞化学中，Triton X-100常用浓度为1%和0.3%，1%的Triton X-100常用于漂洗组织标本，0.3% 的Triton X-100 则常用于稀释血清，配制BSA等。通常是先配制成30%的Triton X-100储存液，临用时稀释至所需浓度。
30%Triton X-100 的配制： Triton X-100 30ml+70mlpbs或者水等。
配制方法：取Triton X-100 及 PBS(或 TBS)混合，置37~40℃水浴中2~3小时，使其充分溶解混匀。用前取该储备液稀释至所需浓度。

实验参考
动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。

动物实验方案计算器

请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。

mg/kg
g 
uL
只
该动物实验的总需药量为 mg
工作液终浓度2为 mg/mL

1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。

其他相关的Solvents & Excipients产品
参考文献

[1] Harendra S, et al. J Environ Sci. Sorption and transport studies of cetyl trimethylammonium bromide (CTAB) and Triton X-100 in clayey soil.

[2] Zhao B, et al. Water Sci Technol. Micellar-enhanced ultrafiltration of copper ions using sodium dodecyl sulfate and its mixture with Brij 35, Tween 80 and Triton X-100.

以上参考文献由AI整理，仅供参考，AbMole 尚未独立确认这些文献的准确性。






关键词：Triton X-100, Triton X-100供应商, Solvents & Excipients抑制剂, 购买Triton X-100, Triton X-100溶解度, Triton X-100结构式




 
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