TMPD dihydrochloride 别名：N,N,N′,N′-四甲基对苯二胺盐酸盐
TMPD dihydrochloride（N,N,N′,N′-四甲基对苯二胺盐酸盐，四甲基对苯二胺二盐酸盐）是一种用于研究细胞色素c的膜栓系化合物，用于研究其对酵母细胞死亡的影响。TMPD dihydrochloride 也是一种 complex IV 的底物。
CAS No.：637-01-4
化学性质/溶解性/储存
| 分子量 | 237.17 |
| 分子式 | C10H18Cl2N2 |
| CAS号 | 637-01-4 |
| 中文名称 | N,N,N′,N′-四甲基对苯二胺盐酸盐；四甲基对苯二胺二盐酸盐 |
| 溶解性 | Water ≥ 60 mg/mL DMSO ≥ 10 mg/mL |
| 储存条件 | 4°C, protect from light, dry, sealed |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
实验参考
下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.2164 mL | 21.0819 mL | 42.1638 mL |
| 5 mM | 0.8433 mL | 4.2164 mL | 8.4328 mL |
| 10 mM | 0.4216 mL | 2.1082 mL | 4.2164 mL |
*吸湿的DMSO对产品的溶解度有显著影响，请使用新开封的DMSO；
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
| 细胞系 | Escherichia coli & Bacillus subtilis |
| 方法 | Bacteria culture and oxidase test Escherichia coli Bacteria were cultured in 2 × TY liquid microbial growth medium (broth), containing 16 g/L tryptone, 10 g/L yeast extract and 5.0 g/L NaCl. Growth medium was inoculated with bacteria from frozen stocks and incubated in glass culture flasks for 18 h at 37 °C in an incubator shaker. An E. coli suspension of 50 μL was transferred into a new culture flask, containing fresh growth medium. Following incubation for 3 to 4 h at 37 °C, the number of bacteria in solution was determined by optical density (OD) at a wavelength of 600 nm (OD600 of 1.0 = 8 × 108 cells/mL). When an OD600 between 0.4 and 1.8 was reached, bacteria were harvested by centrifugation for 15 min at 3000 rcf and re-suspended in pre-warmed (37 °C) PBS. Bacillus subtilis (strain PY79) Low salt growth medium (broth), containing 10 g/L tryptone, 5.0 g/L yeast extract and 5.0 g/L sodium chloride, was inoculated with cultures from frozen stocks and incubated at 30 °C for 36 h in an incubator shaker. An OD600 was determined to calculate number of bacteria in solution (OD600 of 1.0 = 5 × 108 cells/mL). Bacteria were harvested by centrifugation for 10 min at 300 rcf and re-suspended in pre-warmed (30 °C) PBS. Cultures grown on agar, containing 10 g/L tryptone, 5.0 g/L yeast extract 5.0 g/L sodium chloride and 20 g/L agar, were inoculated onto agar plates and grown at 30 °C for 36 h in a static incubator. For the oxidase test, 200 μL of a 1% (wt) N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD-2HCl) solution was added to 1 mL of cell suspension (1 × 107 cells/μL). Images were taken within 30 s of solution mixture. |
| 浓度 | 200 μL of a 1% (wt) N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD-2HCl) solution |
| 处理时间 | - |
* 上述方法来自公开文献，仅供相同目的实验参考。如实验目的、材料、方法不同，请参考其他文献。
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
| 动物模型 | |
| 配制 | |
| 剂量 | |
| 给药处理 |
* 上述方法来自公开文献，仅供相同目的实验参考。如实验目的、材料、方法不同，请参考其他文献。
其他相关的产品
参考文献
