Recombinant Rat VEGF164 (CHO) 别名：Vascular Endothelial Growth Factor A164; VEGF-A164; 重组大鼠VEGF164/VEGF-A (CHO)
Vascular endothelial growth factor (VEGF/VEGF-A) is originally known as vascular permeability factor (VPF). VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties. It belongs to the PDGF family with a cysteine-knot structure comprised of eight conserved cysteine residues, and reckoned as a potent mediator in the process of angiogenesis and vasculogenesis in either fetus or adult.
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组大鼠VEGF164/VEGF-A (CHO) |
| 溶解性 | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Vascular endothelial growth factor (VEGF/VEGF-A) is originally known as vascular permeability factor (VPF). VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties. It belongs to the PDGF family with a cysteine-knot structure comprised of eight conserved cysteine residues, and reckoned as a potent mediator in the process of angiogenesis and vasculogenesis in either fetus or adult. Rat VEGF164 respectively displays 97% and 88% aa identity with that regions of mouse and human VEGF. The active form of rVEGF-A164 is either a homodimeric or heterodimeric polypeptides which bind to the transmembrane tyrosine kinases receptors FLT1, FLK1 or KDR or to the non-tyrosine kinase neuropilin receptors NRP1/2.
Recombinant Rat Vascular Endothelial Growth Factor A expressed the target gene encoding Ala27-Arg190(Ala36Thr).
Measured in a cell proliferation assay using HUVEC cells, the ED50 is < 4.0 ng/ml, corresponding to a specific activity of >2.5 × 105 units/mg.
Accession: P16612-2
Apparent Molecular Weight: 35~48 kDa, on SDS-PAGE under non-reducing conditions
Endotoxin < 0.2 EU/µg
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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