利用大肠杆菌表达系统制备了重组小鼠Sonic Hedgehog基因，并表达了编码Cys25-Gly198的目的基因。Accession #: Q62226。
别名：Sonic Hedgehog Protein; SHH; HHG-1; SHH
种属：Mouse
表达系统: E.coli
纯度：还原SDS-PAGE测定大于95%
内毒素：经LAL测定，小于0.001 ng/ug (0.01 EU/ug)
存储及稳定性：冻干蛋白应在< -20°C保存，但在室温下可保存3周。重组蛋白溶液可在4-7℃下保存2-7天。重组样品的等分液在< -20°C下稳定3个月。
生物活性：小鼠Sonic Hedgehog Homolog (SHH)属于刺猬(Hedgehog)三蛋白家族。其他两个家族成员是印度刺猬(IHH)和沙漠刺猬(DHH)。刺猬蛋白是胚胎发育的关键信号分子。SHH在多种胚胎组织中表达，在调节肢体和大脑等许多系统的模式方面发挥着关键作用。SHH在成人中也起着重要作用，包括成人干细胞的分裂以及某些癌症和其他疾病的发生。小鼠Shh合成为437aa前体，包含一个24aa信号序列和一个413aa成熟区。成熟区域被自催化加工成一个非糖基化，20kda, 174 aa n端片段(sh - n)和一个催化加工，34 kDa, 239 aa c端片段。20 kDa的Shh-N片段是活性hedgehog分子的核心。小鼠Shh-N与人Shh-N、大鼠Shh-N和沙鼠Shh-N的同源性分别为99%、98%和100%。
| 溶解性（25°C） | It is not recommended to reconstitute to a concentration less than 100 μg/ml. |
| 储存条件 |
粉末型式 -20°C 3年；4°C 2年 溶于溶剂 -80°C 6个月；-20°C 1个月 |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
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蛋白质研究