Recombinant Mouse SDF-1 Protein (E. coli) 别名：Stromal cell-derived factor 1; CXCL12; 重组小鼠SDF-1蛋白 (E. coli)
重组小鼠SDF-1蛋白又称 CXCL12，是一种高度保守的小型趋化细胞因子（8 kDa），属于 CXC 趋化因子大家族。SDF-1 主要与 CXC 受体 4（CXCR4；CD184）结合。CXCL12 与 CXCR4 结合后，能通过几种不同的途径诱导细胞内信号传导，启动与趋化、细胞存活和/或增殖、细胞内钙增加和基因转录有关的信号。
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组小鼠SDF-1蛋白 (E. coli) |
| 溶解性 | Reconstitute the lyophilized powder in sterile water up to 150 μg/ml. |
| 储存条件 | Stable for up to twelve months from date of receipt at -20℃ to -80℃ |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
The stromal cell-derived factor-1 (SDF1), also known as CXCL12, is a small (8 kDa) cytokine highly conserved chemotactic cytokine belonging to the large family of CXC chemokines. SDF-1 binds primarily to CXC receptor 4 (CXCR4; CD184). The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription. SDF-1/CXCL12 has crucial roles in the formation of multiple organ systems during embryogenesis and in the regulation of bone marrow haematopoiesis and immune function in the postnatal organism.
A DNA sequence encoding the mature form of mouse SDF-1 (Lys22-Lys89) was expressed and purified with an initial Met.
The recombinant mouse SDF-1 consists of 69 amino acids and predicts a molecular mass of 8.1 KDa. It migrates as an approximately 8 KDa band in SDS-PAGE under reducing conditions.
Accession: P40224
Endotoxin: < 1.0 EU per μg protein as determined by LAL test
Lyophilized from sterile 50% acetonitrile, 1% TFA.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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