Recombinant Mouse Interleukin-4是一种多效性细胞因子,可调节多种T和B细胞反应,包括细胞增殖、存活和基因表达。IL-4 调节天然 CD4+ T 细胞(Th0 细胞)向辅助 Th2 细胞的分化,并调节免疫球蛋白类别转换为 IgG1 和 IgE 同种型。
中文名称 | 重组小鼠白细胞介素-4 |
溶解性 | Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml. |
储存条件 | Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
Recombinant Mouse Interleukin-4 is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It is produced by mast cells, activated T cells and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells.
ED50 < 2 ng/ml, measured in a cell proliferation assay using murine HT-2 cells, corresponding to a specific activity of >5 x 105 units/mg.
Accession: # P07750
Apparent Molecular Weight: ~15 kDa, on SDS-PAGE under reducing conditions.
Lyophilized after extensive dialysis against PBS.
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。