Interleukin-2/IL-2 is a secreted protein that belongs to the IL-2 family. Interleukin-2/IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
性状 | Lyophilized powder |
中文名称 | 重组小鼠IL-2蛋白 (E. coli) |
溶解性 | Reconstitute the lyophilized powder in ddH2O or PBS to 100 μg/ml. |
储存条件 | Stored at ≤-20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
Interleukin-2/IL-2 is a secreted protein that belongs to the IL-2 family. Interleukin-2/IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Interleukin-2 / IL-2 can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
The recombinant mouse IL2 consists of 150 amino acids and has a calculated molecular mass of 17.4KDa.
A DNA sequence encoding the soluble form of mouse IL2 (P04351) (Ala 21-Gln 169) was expressed, with an initial Met at the C-terminus.
Measured by cell proliferation assay using CTLL2, the ED50 for this effect is typically 6-30 ng/mL.
Accession: P04351
Molecular Weight: Approximately 19 kDa in SDS-PAGE under reducing conditions
Lyophilized from sterile PBS, 2mM DTT, pH 7.4.
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。