表达系统:E. coli 生物活性:ED50 < 5.0 pg/ml,在小鼠FDC-P1细胞的细胞增殖实验中测量,对应的比活性为> 2.0 × 108 units/mg。
别名:Granulocyte Macrophage Colony Stimulating Factor; CSF-2; MGI-1GM
纯度:> 98% SDS-PAGE分析, HPLC分析结果为98%
内毒素:凝胶凝血法测定蛋白质< 0.2 EU/μg
表观分子量:~14.3 kDa,在SDS-PAGE还原条件下。
存储及稳定性:在接收后,该产品在低于-70°C的环境下保持稳定长达6个月。重组后,产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存,建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环。
生物活性:粒细胞巨噬细胞集落刺激因子(GM-CSF)最初被认为是一种能够支持粒细胞-巨噬细胞祖细胞体外集落形成的生长因子。粒细胞巨噬细胞集落刺激因子(GM-CSF)由许多不同类型的细胞产生,包括活化的T细胞、B细胞、巨噬细胞、肥大细胞、内皮细胞和成纤维细胞,对免疫和炎症刺激的细胞因子做出反应。此外,粒细胞巨噬细胞集落刺激因子(GM-CSF)可以刺激多种肿瘤细胞系的增殖,包括骨肉瘤、癌和腺癌细胞系。
溶解性(25°C) | Reconstitute the lyophilized powder in ddH₂O or PBS to 100 μg/ml |
储存条件 | Stable for up to 6 months at lower than -70°C |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
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