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Recombinant Mouse GM-CSF Protein (E. coli) 重组小鼠GM-CSF蛋白(E. coli)

目录号 M11485 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


表达系统:E. coli 生物活性:ED50 < 5.0 pg/ml,在小鼠FDC-P1细胞的细胞增殖实验中测量,对应的比活性为> 2.0 × 108 units/mg。

Recombinant Mouse GM-CSF Protein (E. coli)结构式

别名:Granulocyte Macrophage Colony Stimulating Factor; CSF-2; MGI-1GM

规格 价格 库存状态
10ug ¥ 1770 中国库存现货
50ug ¥ 4320 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >98%, Endotoxin < 0.2 EU/μg
  • COA
  • MSDS
生物活性

纯度:> 98% SDS-PAGE分析, HPLC分析结果为98%

内毒素:凝胶凝血法测定蛋白质< 0.2 EU/μg

表观分子量:~14.3 kDa,在SDS-PAGE还原条件下。

存储及稳定性:在接收后,该产品在低于-70°C的环境下保持稳定长达6个月。重组后,产品应在4°C下稳定1周或在-20°C下稳定3个月。为了长期保存,建议添加载体蛋白(如0.1% BSA)。避免反复的冻融循环。


生物活性:粒细胞巨噬细胞集落刺激因子(GM-CSF)最初被认为是一种能够支持粒细胞-巨噬细胞祖细胞体外集落形成的生长因子。粒细胞巨噬细胞集落刺激因子(GM-CSF)由许多不同类型的细胞产生,包括活化的T细胞、B细胞、巨噬细胞、肥大细胞、内皮细胞和成纤维细胞,对免疫和炎症刺激的细胞因子做出反应。此外,粒细胞巨噬细胞集落刺激因子(GM-CSF)可以刺激多种肿瘤细胞系的增殖,包括骨肉瘤、癌和腺癌细胞系。

化学性质
溶解性(25°C) Reconstitute the lyophilized powder in ddH₂O or PBS to 100 μg/ml
储存条件 Stable for up to 6 months at lower than -70°C
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] Elektra Kantzari Robinson, et al. PLoS One. Generation and utilization of a HEK-293T murine GM-CSF expressing cell line

[2] Michael Dougan, et al. Immunity. GM-CSF, IL-3, and IL-5 Family of Cytokines: Regulators of Inflammation

[3] Marco Grasse, et al. Vaccine. GM-CSF improves the immune response to the diphtheria-component in a multivalent vaccine

[4] Qingjie Xue, et al. Hum Vaccin Immunother. Efficacy of recombinant adenovirus expressing a fusion gene from GM-CSF and Epstein-Barr virus LMP2A in a mouse tumor model

[5] B L Liu, et al. Gene Ther. ICP34.5 deleted herpes simplex virus with enhanced oncolytic, immune stimulating, and anti-tumour properties

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