Recombinant Mouse CCL21 Protein (E. coli) 别名：C-C Motif Chemokine 21a; 重组小鼠CCL21蛋白 (E. coli)
C-C Motif Chemokine 21a (CCL21) is a small secreted cytokine that belongs to the intercrine beta (CC chemokine) family. Mouse CCL21 inhibits hemopoiesis and stimulates chemotaxis. It has chemotactic function in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils.
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组小鼠CCL21蛋白 (E. coli) |
| 溶解性 | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
C-C Motif Chemokine 21a (CCL21) is a small secreted cytokine that belongs to the intercrine beta (CC chemokine) family. Mouse CCL21 inhibits hemopoiesis and stimulates chemotaxis. It has chemotactic function in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Mouse CCL21 shows preferential activity towards naive T-cells and may play a role in mediating homing of lymphocytes to secondary lymphoid organs.
Mouse CCL21 cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Recombinant Mouse C-C Motif Chemokine 21a expressed the target gene encoding Ser24-Gly133.
Accession: P84444
Endotoxin < 1 EU/µg
Apparent Molecular Weight: 16 KDa, reducing conditions
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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参考文献
