VEGF 121蛋白即血管内皮生长因子121,是血管内皮生长因子(VEGF)家族中的一种亚型。VEGF 121蛋白可以刺激内皮细胞增殖、存活,促进血管生成和血管通透性增加。
性状 | Lyophilized powder |
中文名称 | 重组人VEGF 121 蛋白 (E.coli) |
溶解性 | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
Human VEGF121 belongs to the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family. VEGF121 is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, permeabilization of blood vessels and endothelial cell growth, increasing microvascular permeability, promoting cell migration and inhibiting apoptosis. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.
Immobilized Human VEGF 121 at 2μg/ml (100 μl/well) can bind Human VEGFR2-Fc, the ED50 of Human VEGFR2-Fc is 13.04 ng/ml.
Recombinant Human VEGF121 expressed the target gene encoding Ala27-Arg147.
Accession: P15692-9
Endotoxin < 1 EU/µg
Apparent Molecular Weight: 16 KDa, reducing conditions
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。