重组人转化生长因子β 1(TGFβ-1)是一种分泌蛋白,属于TGF-β家族。TGFβ-1具有多种细胞功能,包括控制细胞生长、细胞增殖、细胞分化和细胞凋亡。以抑制IL-4依赖性的TF-1人类红血病细胞增殖的能力来测量,其ED50值为4-40 pg/ml。
中文名称 | 重组人TGF-beta 1蛋白 (HEK293) |
溶解性 | Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
Recombinant Human TGF-beta 1 (TGFβ-1) performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members.
Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells, the ED50 is 4-40 pg/ml.
Recombinant Human Transforming Growth Factor Beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed.
Accession: P01137
APMol Mass: 13 KDa, reducing conditions
Endotoxin: < 0.01 EU/µg as determined by LAL test
Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5.
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。