Recombinant Human R-spondin-1/RSPO1 Protein (HEK293, C-6His) 别名：重组人R-spondin-1/RSPO1蛋白 (HEK293, C-6His)
R-spondin-1/RSPO1是一种分泌蛋白，含有2个FU（呋喃样）重复序列和1个TSP-1型结构域，属于R-spondin家族。重组人 R-spondin-1 由哺乳动物表达系统生产，目标基因编码为 Ser21-Ala263，C-末端带有 6His 标记。
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人R-spondin-1/RSPO1蛋白 (HEK293, C-6His) |
| 溶解性 | Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
R-spondin-1/RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 augments the WNT/β catenin pathway to oppose male sex development. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. Recombinant Human R-spondin-1 is produced by Mammalian expression system, and the target gene encoding Ser21-Ala263 is expressed with a 6His tag at the C-terminus.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells, the ED50 for this effect is 4.06 ng/ml.
Accession: Q2MKA7
APMol Mass: 40 KDa, reducing conditions
Endotoxin < 0.01 EU/µg
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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参考文献
