Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
别名:Proliferating Cell Nuclear Antigen
Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31 kDa.
A DNA sequence encoding the mature form of human PCNA (Met 1-Ser 261) was expressed, with a polyhistidine tag at the N-terminus.
Endotoxin < 1.0 EU/μg
Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10% glycerol, pH 7.0, 2mM DTT.
性状 | Lyophilized powder |
储存条件 | Stored at ≤ -20°C, stable for up to one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
[1] Li Fan et al. Biochem J. DNA-damage tolerance through PCNA ubiquitination and sumoylation
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