Recombinant Human PCNA Protein (Baculovirus-Insect, His) 别名：Proliferating Cell Nuclear Antigen; 重组人PCNA蛋白 (Baculovirus-Insect, His)
Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人PCNA蛋白 (Baculovirus-Insect, His) |
| 储存条件 | Stored at ≤ -20°C, stable for up to one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Proliferating Cell Nuclear Antigen (PCNA) is a protein only expressed in normal proliferate cells and cancer cells. One of the well-established functions for PCNA is its role as the processivity factor for DNA polymerase delta and epsilon. PCNA tethers the polymerase catalytic unit to the DNA template for rapid and processive DNA synthesis. The close association of PCNA with kinase complexes involved in cell cycle machinery indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31 kDa.
A DNA sequence encoding the mature form of human PCNA (Met 1-Ser 261) was expressed, with a polyhistidine tag at the N-terminus.
Endotoxin < 1.0 EU/μg
Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10% glycerol, pH 7.0, 2mM DTT.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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