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Recombinant Human Midkine (E. coli) 重组人Midkine蛋白 (E. coli)

目录号 M25412 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. Midkine promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis.

Recombinant Human Midkine (E. coli)结构式

别名:Midgestation and Kidney Protein

规格 价格 库存状态
5ug ¥ 1280 中国库存现货
100ug ¥ 9600 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >97%, Endotoxin <1EU/μg
  • COA
  • MSDS
生物活性

Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. Midkine promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis.

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.


Accession: P21741

Approximately Molecular Weight: 13.4 kDa, a single non-glycosylated polypeptide chain containing 123 amino acids.

AA Sequence:
VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

化学性质
溶解性(25°C) Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.
储存条件 Stable for up to twelve months from date of receipt at -20℃ to -70℃. Reconstituted protein solution can be stored at 2-8°C for 2 weeks, at ≤ -20°C for 3 months.
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] Priyo K Singh, et al. Protein Expr Purif. Recombinant expression and purification of heparin binding proteins: midkine and pleiotrophin from Escherichia coli

[2] W Kelly Yan, et al. Protein Expr Purif. High-level soluble expression, purification and characterization of active human midkine from Escherichia coli

[3] Zhong-hui Zhang, et al. J Zhejiang Univ Sci B. Expression and purification of bioactive high-purity human midkine in Escherichia coli

[4] Qing-ling Wang, et al. Wei Sheng Yan Jiu. [Cloning and expression of truncated Midkine cytokine from gastric carcinogenesis tissue in E. coli]

[5] H Maruta, et al. Growth Factors. Midkine (MK), a retinoic acid (RA)-inducible gene product, produced in E. coli acts on neuronal and HL60 leukemia cells

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