Recombinant Human Midkine (E. coli) 别名：Midgestation and Kidney Protein; 重组人Midkine蛋白 (E. coli)

目录号 M25412

Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. Midkine promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis.

规格	价格	库存状态
5ug	¥ 1280	现货
100ug	¥ 9600	现货

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质量标准及产品资料

纯度：>97%, Endotoxin <1EU/μg
COA

化学性质/溶解性/储存

中文名称	重组人Midkine蛋白 (E. coli)
溶解性	Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.
储存条件	Stable for up to twelve months from date of receipt at -20℃ to -70℃. Reconstituted protein solution can be stored at 2-8°C for 2 weeks, at ≤ -20°C for 3 months.
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

生物活性

The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.

Accession: P21741

Approximately Molecular Weight: 13.4 kDa, a single non-glycosylated polypeptide chain containing 123 amino acids.

AA Sequence:
VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

实验参考

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。