Recombinant Human IL-34 Protein (CHO, C-His) 别名：Interleukin 34; 重组人IL-34蛋白 (CHO, C-His)
IL-34 是一种细胞因子，可通过集落刺激因子-1 受体 (CSF1R) 促进单核细胞和巨噬细胞的分化和活力。使用人外周血单核细胞在细胞增殖试验中测得的 ED50 为 2-8 ng/mL。
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人IL-34蛋白 (CHO, C-His) |
| 溶解性 | Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
IL-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R). IL34 (Interleukin 34) is a Protein Coding gene. IL-34, also known as uncharacterized protein C16 or f77 homolog, belongs to the IL-34 family. IL-34 increases the growth or survival of immune cells known as monocytes. Besides, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures. IL-34 protein is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and most abundant in the spleen.
The recombinant human IL34 consists of 233 amino acids and predicts a molecular mass of 26.7 kDa. A DNA sequence encoding the human IL34 (AAH29804.1) (Met1-Pro242) was expressed with a polyhistidine tag at the C-terminus.
Lyophilized from sterile PBS, pH 7.4.
Measured in a cell proliferation assay using human peripheral blood mononuclear cell, the ED50 is 2-8 ng/mL.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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参考文献
