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Recombinant Human IFN alpha2a (E.coli) 重组人 IFN alpha2a蛋白(E.coli)

目录号 M20003 所有产品仅供科研使用,严禁用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务  


重组人干扰素Alpha-2a由大肠杆菌表达系统生产。蛋白编号:P01563。ED50 < 0.1 ng/ml,通过使用TF-1细胞的细胞毒性试验测量,对应的特异性活性> 1.0 × 107 units/mg。

Recombinant Human IFN alpha2a (E.coli)结构式

别名:Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A

规格 价格 库存状态
10ug ¥ 747 中国库存现货
50ug ¥ 1350 中国库存现货
其他规格数量报价?

质量标准及产品资料
  • 纯度: >95% , Endotoxin < 1 EU/µg
  • COA
  • MSDS
生物活性

种属:Human

表达系统:E.coli

纯度: 通过还原SDS-PAGE测定,大于95%

内毒素:经LAL测试,小于0.1 ng/µg(1 EU/µg)

表观分子量:16 KDa,还原条件

存储及稳定性:冻干的蛋白质应储存在<-20°C,尽管在室温下可稳定3周。重组的蛋白质溶液可在4-7°C储存2-7天。重组的样品等分在<-20°C下可稳定3个月。


生物活性:由白细胞产生的人类干扰素Alpha 2a(IFN-Alpha 2a)是干扰素家族的一个成员。IFN-α主要参与先天性免疫反应,以对抗广泛的病毒感染。IFN-alpha 2有三种酸性稳定形式(a,b,c),通过IFNAR2进行信号传递。IFN-alpha 2a与IFN-alpha 2b和2c分别有99.4%、98.8%的相同序列。IFN-alpha含有四个高度保守的半胱氨酸残基,形成两个二硫键,其中一个是生物活性所必需的。

化学性质
溶解性(25°C) It is not recommended to reconstitute to a concentration less than 100μg/ml.
储存条件 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
参考文献

[1] Paulo V S Dias, et al. J Chromatogr B Analyt Technol Biomed Life Sci. Determination of recombinant Interferon-α2 in E. coli periplasmic extracts by reversed-phase high-performance liquid chromatography

[2] Y Mohammed, et al. Biochemistry (Mosc). Expression of human interferon-α8 synthetic gene under P(BAD) promoter

[3] J Meng, et al. Cytotherapy. High-yield expression, purification and characterization of tumor-targeted IFN-alpha2a

[4] Zhen Yan, et al. Appl Biochem Biotechnol. Expression, refolding, and characterization of GFE peptide-fused human interferon-alpha2a in Escherichia coli

[5] E Baron, et al. Crit Rev Biotechnol. From cloning to a commercial realization: human alpha interferon

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关键词:Recombinant Human IFN alpha2a (E.coli), Interferon Alpha-2; IFN-Alpha-2; Interferon Alpha-A, Recombinant Human IFN alpha2a (E.coli)供应商, Recombinant Proteins抑制剂, 购买Recombinant Human IFN alpha2a (E.coli), Recombinant Human IFN alpha2a (E.coli)溶解度, Recombinant Human IFN alpha2a (E.coli)结构式








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