Recombinant Human GSK-3 beta Protein (Baculovirus-Insect, His Tag) 别名：GSK3B; glycogen synthase kinase 3 beta; 重组人GSK-3 beta 蛋白 (Baculovirus-Insect, His Tag)
GSK-3 beta/GSK3B Protein is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB, and the transcription factor JUN.
化学性质/溶解性/储存
| 性状 | Solution |
| 中文名称 | 重组人GSK-3 beta 蛋白 (Baculovirus-Insect, His Tag) |
| 储存条件 | Stable for up to twelve months from date of receipt at -20℃ to -80℃. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
GSK-3 beta/GSK3B Protein is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB, and the transcription factor JUN. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which are critical for its role in bulge stem cell migration and skin wound repair.
The recombinant human GSK3B consists of 452 amino acids and predicts a molecular mass of 50.4 kDa. It expressed the target gene encoding (Met 1-Thr 433) with a polyhistidine tag at the N-terminus.
Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC50 of biotinylated human HG3C-CTNNB1 is 0.15-0.35 μg/ml.
Endotoxin < 1 EU/µg
Apparent Molecular Weight: 44-48 kDa, reducing conditions
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 25% glycerol, 0.5mM PMSF, 0.5mM EDTA.
Shipping with dry ice.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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