Recombinant Human Granzyme B Protein (HEK293, C-His) 别名：CGL-1; CSP-B; GZMB; 重组人Granzyme B蛋白 (HEK293, C-His)

目录号 M45089

Granzyme B is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells.

Recombinant Human Granzyme B Protein (HEK293, C-His)结构式

规格	价格	库存状态
50ug	¥ 8100	现货

*AbMole所有产品仅供有资质的科研机构或医药企业进行科学研究或药证申报用途，不能被用于人体和任何其它用途。我们不向任何个人或非科研性质的机构提供产品和服务。

质量标准及产品资料

纯度：>97%, Endotoxin < 1 EU/µg
COA

化学性质/溶解性/储存

性状	Lyophilized powder
中文名称	重组人Granzyme B蛋白 (HEK293, C-His)
溶解性	Reconstitute the lyophilized powder in distilled water up to 100 μg/ml.
储存条件	Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months.
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

生物活性

Granzyme B is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Granzyme B (GZMB) protects the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.

The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27.2 kDa. A DNA sequence encoding the proform of human Granzyme B (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.

Endotoxin < 1 EU/µg

Apparent Molecular Weight: 35-40 kDa, reducing conditions

Lyophilized from sterile PBS, pH 7.4.

实验参考

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。