Recombinant Human Beta-NGF Protein (E. coli)  别名:β-NGF; NGFB; Beta-Nerve Growth Factor; 重组人Beta-NGF蛋白 (E. coli)

目录号 M31178

Human β-Nerve Growth Factor (β-NGF) is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival.

Recombinant Human Beta-NGF Protein (E. coli)结构式

 

规格 价格 库存状态
10ug ¥ 1044 现货
50ug ¥ 2790 现货
*AbMole所有产品仅供有资质的科研机构或医药企业进行科学研究或药证申报用途,不能被用于人体和任何其它用途。我们不向任何个人或非科研性质的机构提供产品和服务。
质量标准及产品资料
  • 纯度:>95%, Endotoxin < 0.01 EU/μg
  • COA
化学性质/溶解性/储存
性状 Lyophilized powder
中文名称 重组人Beta-NGF蛋白 (E. coli)
溶解性 Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml.
储存条件 Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at ≤ -20°C for 3 months.
运输方式 冰袋运输,根据产品的不同,可能会有相应调整。
生物活性

Human β-Nerve Growth Factor (β-NGF) is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity. 

Recombinant Human Beta-NGF is produced by E.coli expression system and expressed the target gene encoding Ser122-Ala241.

Measured by the cell proliferation assay using TF‑1 human erythroleukemic cells, the ED50 is 0.03-0.3 ng/ml.


Accession: P01138

APMol Mass: 14 KDa, reducing conditions

Endotoxin < 0.01 EU/µg

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

实验参考
动物实验

建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。


动物实验方案计算器

请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。

mg/kg
uL
该动物实验的总需药量为 mg
工作液终浓度2 mg/mL

1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。






关键词:Recombinant Human Beta-NGF Protein (E. coli), β-NGF; NGFB; Beta-Nerve Growth Factor, Recombinant Human Beta-NGF Protein (E. coli)供应商, Cytokines and Growth Factors抑制剂, 购买Recombinant Human Beta-NGF Protein (E. coli), Recombinant Human Beta-NGF Protein (E. coli)溶解度, Recombinant Human Beta-NGF Protein (E. coli)结构式







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