Recombinant Human Interleukin-33 (E. coli) 别名：rHuIL-33; IL-1F11; 重组人白介素-33 (E. coli)
白细胞介素-33 (IL-33) 是一种促炎细胞因子，属于 IL-1 家族。IL-33 在多种细胞中表达，包括上皮细胞和内皮细胞、平滑肌细胞、巨噬细胞和成纤维细胞。IL-33 的主要受体是 ST2 和 IL-1 受体辅助蛋白 (IL-1RAcP)，IL-33 在损伤过程中由细胞分泌，诱导 T 辅助 2 型炎症反应。
化学性质/溶解性/储存
| 性状 | Lyophilized powder |
| 中文名称 | 重组人白介素-33 (E. coli) |
| 溶解性 | Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml. |
| 储存条件 | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| 运输方式 | 冰袋运输，根据产品的不同，可能会有相应调整。 |
生物活性
Human Interleukin-33 (IL-33) is a proinflammatory cytokine that belongs to the IL-1 family. IL-33 plays a role in autoimmune disease. IL-33’s interaction with ST2 can drive allergic pathology and IL-33 has been reported to play a role in the development of rheumatoid arthritis and systemic lupus erythematosus. IL-33 is expressed in a variety of cells, including epithelial and endothelial cells, smooth muscle cells, macrophages and fibroblasts. The primary receptors for IL-33 are ST2 and IL-1 receptor accessory protein (IL-1RAcP), both of which belong to the IL-1 receptor family. IL-33 is localized to the nucleus of resting cells where it binds to chromatin in the H2A-H2B histone complex as a transcriptional suppressor. IL-33 is secreted by cells during injury which induces a T-helper 2 type inflammatory response.
ED50 < 0.5 ng/ml, measured by a cell proliferation assay using D10S cells, corresponding to a specific activity of > 2.0 × 106 units/mg.
Recombinant Human Interleukin-33 (IL-33) expressed the target gene encoding Ser112-Thr270.
Accession: O95760
Endotoxin < 0.2 EU/µg
Apparent Molecular Weight: ~18.0 kDa, reducing conditions
Lyophilized after extensive dialysis against PBS.
实验参考
建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中，输入您的动物实验相关数据并点击计算，即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg，平均每只动物的体重为20 g，每只动物的给药体积是100 μL，动物数量为20只，则该动物实验的总需药量为4 mg，工作液终浓度为2 mg/mL。
1：鉴于实验过程的损耗，建议您至少多配1-2只动物的量；
2：为该产品最终给药时的浓度。
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