H2DCFDA 别名：DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate; 活性氧(ROS)探针

目录号 M9096

H2DCFDA是一种可渗透细胞，并检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm)，可用于细菌活性氧（ROS）产生的测定。

CAS No.：4091-99-0

规格	价格	库存状态
10mg	¥ 234	现货
25mg	¥ 360	现货
50mg	¥ 450	现货

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质量标准及产品资料

纯度：99.72%
COA
H-NMR
HPLC

化学性质/溶解性/储存

分子量	487.29
分子式	C24H16Cl2O7
CAS号	4091-99-0
中文名称	活性氧(ROS)探针
溶解性	DMSO: ≥ 90 mg/mL
储存条件	-20°C, protect from light
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

生物活性

H2DCFDA是可渗透细胞，用于检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm)。H2DCFDA可以用于细菌活性氧（ROS）产生的测定。

ROS测量

将H2DCFDA溶解在DMSO中以获得10mM储备溶液，并在使用前进一步稀释。

为了检测细胞内ROS水平，使用ROS敏感性探针H2DCFDA。将贴壁细胞（ESC，difESC，eMSC，HeLa，U118）与PBS中的5μM染色溶液在37℃下在黑暗中孵育30分钟，然后用0.05%胰蛋白酶-EDTA溶液收获，悬浮在新鲜培养基中，并且用流式细胞仪立即分析。将对照和PHA活化的淋巴细胞重悬浮于PBS中，与5μM H2DCFDA在黑暗中于37℃温育30分钟，并立即分析。

实验参考

蛋白/细胞实验

下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	2.0522 mL	10.2608 mL	20.5217 mL
5 mM	0.4104 mL	2.0522 mL	4.1043 mL
10 mM	0.2052 mL	1.0261 mL	2.0522 mL

*吸湿的DMSO对产品的溶解度有显著影响，请使用新开封的DMSO；
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

质量		浓度		体积		分子量*
	=		x		x

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。