Anti-Mouse Ly-6G/Ly-6C Antibody (Clone RB6-8C5) In vivo has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo.
储存条件 |
Stored sterile as received at 2-8°C for up to one month. For longer term storage, store at -80°C without diluting. Avoid Repeated Freeze Thaw Cycles. |
运输方式 | 冰袋运输,根据产品的不同,可能会有相应调整。 |
Anti-Mouse Ly-6G/Ly-6C Antibody (Clone RB6-8C5) In vivo has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody.
FC: The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 μg per 106 cells in a volume of 100 μl or 100 μl of whole blood.
Clone:RB6-8C5
Formulation:PBS pH 7.2 - 7.4
Reactivity Species:Mouse
Host Species:Rat
Product Concentration:≥ 5.0 mg/ml
Endotoxin:< 1.0 EU/mg
Purity:≥95%
建议您制定动物给药及实验方案时,尽量参考已发表的相关实验文献(溶剂种类及配比众多,简单地溶解目的化合物,并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题,未必能保证目的化合物在动物体内充分发挥生物学效用)。
体内实验的工作液,建议您现用现配,当天使用;如在配制过程中出现沉淀、析出现象,可以通过超声和(或)加热的方式助溶。
切勿一次性将产品全部溶解。
请在下面的计算器中,输入您的动物实验相关数据并点击计算,即可得到该实验的总需药量和工作液终浓度。
例如您给药剂量是10 mg/kg,平均每只动物的体重为20 g,每只动物的给药体积是100 μL,动物数量为20只,则该动物实验的总需药量为4 mg,工作液终浓度为2 mg/mL。
1:鉴于实验过程的损耗,建议您至少多配1-2只动物的量;
2:为该产品最终给药时的浓度。