Cell Counting Kit-8试剂盒可谓是目前在细胞实验中炙手可热的一款试剂盒,常用于对细胞的增殖检测和毒性检测。相比较于往常的MTT试剂盒,CCK-8试剂盒的操作更加简便,而且灵敏度更高,因此在科研界CCK-8试剂盒一路走红,广受好评。
自AbMole推出CCK-8试剂盒以来,该产品广受全球好评。自AbMole购买使用CCK-8试剂盒发表的文献多达数十余篇,高质量的文献结果展示出了我们CCK-8试剂盒的精准度与灵敏性。
实验结果展示:
Figure 4. (A) Proliferation and (B) viability were compared among pcDNA3.1-H19-, pcDNA3.-, si-H19-, si-NC-, miR-29b-3p inhibitor-, miR-29b-3p mimic-and negative control-transfected Calu-3 and NCI-H1975 cell lines. *P<0.05 vs. pcDNA3.1; #P<0.05 vs. Si-NC; @P<0.05 vs. negative control. si, short interfering; NC, negative control; miR, microRNA
Figure 8. (A) Colony formation and (B) Cell Counting Kit-8 assays were performed to determine the proliferative capacity and viability of Calu-3 and NCI-H1975 cell lines among miR-NC, miR-29b-3p mimic and miR-NC+STAT3 groups. *P<0.05 vs. miR‑29b‑3p. miR, microRNA; NC, negative control; STAT3, signal transducer and activator of transcription 3.
实验结果展示:
Fig. 4. In vitro testing of the SI-PI-TNs. (A) Hemolysis assay and (B) cytotoxicity of the prepared SI-PI-TNs and corresponding dissolved products at 0.2 mg/mL dose. (C) Fluorescence images of FDA (green; live cells) and PI (red; dead cells) double-stained L-929 cells treated with SI-PI-TNs at 24-72 h of incubation compared with the control group treated with PBS at 72 h of incubation. (D) In vitro drug (IVM) release profiles of TNs with or without PLGA particles.
3. Knockdown of MON1B Exerts Anti-Tumor Effects in Colon Cancer In Vitro
实验结果展示:
Figure 2. MON1B interference inhibited cell proliferation, migration, and invasion abilities of colon cancer cells. The mRNA (A) and protein levels (B) of MON1B decreased remarkably in the si-MON1B group compared with Control and NC groups. (C) Cell proliferation abilities were inhibited in a time-dependent (12, 24, and 48 h) manner in the si-MON1B group compared with Control and NC groups. (D, E) Cell migration abilities of colon cancer cells were inhibited in a time-dependent (12 and 24 h) manner in the si-MON1B group as detected by wound-healing assay. (D, F) Cell invasion abilities of colon cancer cells were significantly also inhibited. * P<0.05 and ** P<0.01 vs. Control group; # P<0.05 and ## P<0.01 vs. NC group.
Figure 3. MON1B interference inhibited cell migration and invasion abilities of colon cancer cells via inhibiting MMPs. (A-D) The mRNA levels of MMP-2, MMP-9, and MTA-1 were inhibited, while that of TIMP-2 was promoted in the si-MON1B group. (E) The protein levels of MMP-2, MMP-9, and MTA-1 were inhibited, while that of TIMP-2 was promoted in the si-MON1B group. * P<0.05 and ** P<0.01 vs. Control group; # P<0.05 and ## P<0.01 vs. NC group.
一篇又一篇的高分文献也在印证着我们cck-8试剂盒的品质,AbMole一直秉持本心,对我们的产品品质都严加把关,确保每一份试剂都合规,让您的每一个实验都无忧。