Actinomycin D 别名：Actinomycin Aiv; Dactinomycin; Act D; RASP-101; 放线菌素D

目录号 M4881

Actinomycin D（RASP-101）是一种从土壤细菌中分离出来的多肽抗生素，同时也是大环类化合物，可嵌入DNA双螺旋的小沟中，与DNA形成复合体，阻碍RNA多聚酶的功能，从而抑制RNA的合成。Actinomycin D 抑制 DNA 修复，IC50 值为 0.42 μM。Actinomycin D 也是一种自噬 (autophagy) 激活剂。

CAS No.：50-76-0

规格	价格	库存状态
5mg	¥ 456	现货
10mg	¥ 840	现货
50mg	¥ 3040	现货

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客户使用AbMole的Actinomycin D发表的文献

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质量标准及产品资料

纯度：99.07%
COA
H-NMR
HPLC

化学性质/溶解性/储存

分子量	1255.41
分子式	C62H86N12O16
CAS号	50-76-0
中文名称	放线菌素D
溶解性	DMSO ≥ 30 mg/mL
储存条件	粉末型式 -20°C 3年；4°C 2年溶于溶剂 -80°C 6个月；-20°C 1个月
运输方式	冰袋运输，根据产品的不同，可能会有相应调整。

生物活性

Actinomycin D（RASP-101）是一种从土壤细菌中分离出来的多肽抗生素。Actinomycin D可抑制DNA的修复和RNA的转录、转运，使细胞周期停滞在G1期。Actinomycin D是自噬的激活剂，可在淋巴细胞性白血病中诱导p53依赖性细胞的死亡。

Actinomycin D (Dactinomycin) 发现于小链霉菌 (Streptomyces microsporum)，具有抗菌和抗肿瘤作用。Actinomycin D 是一种 RNA 和蛋白质合成抑制剂，能够抑制细菌的蛋白合成，可以直接结合转录起始复合体中单链或双链 DNA 的鸟嘌呤基团，抑制 DNA 依赖的 RNA 聚合酶活力，干扰 DNA 的转录过程，从而抑制 mRNA 合成。Actinomycin D 抑制 DNA 修复，IC50 值为 0.42 μM。Actinomycin D 也是一种自噬 (autophagy) 激活剂。

Actinomycin D (0.06 mg/kg; 腹腔注射，连续 14 天) 导致慢性淋巴细胞白血病小鼠模型肿瘤消退。

实验参考

蛋白/细胞实验

下述溶液配置方法仅为基于分子量计算出的理论值。不同产品在配置溶液前，需考虑其在不同溶剂中的溶解度限制。

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	0.7966 mL	3.9828 mL	7.9655 mL
5 mM	0.1593 mL	0.7966 mL	1.5931 mL
10 mM	0.0797 mL	0.3983 mL	0.7966 mL

*吸湿的DMSO对产品的溶解度有显著影响，请使用新开封的DMSO；
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

质量		浓度		体积		分子量*
	=		x		x

动物实验

建议您制定动物给药及实验方案时，尽量参考已发表的相关实验文献（溶剂种类及配比众多，简单地溶解目的化合物，并不能解决动物给药依从性、体内生物利用度、组织分布等相关问题，未必能保证目的化合物在动物体内充分发挥生物学效用）。
体内实验的工作液，建议您现用现配，当天使用；如在配制过程中出现沉淀、析出现象，可以通过超声和（或）加热的方式助溶。
切勿一次性将产品全部溶解。